<<< Full experiment listing

PXD011335

PXD011335 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn vitro phosphorylation of ASY1 by CDKA;1
DescriptionMeiosis is a complex cell division program reducing chromosome number as a prerequisite for sexual reproduction and at the same time leading to a new combination of parental alleles contributing to biodiversity. Little is known about how the distinct order of the molecular processes of meiosis are orchestrated. Here we show that the Arabidopsis Cdk1/Cdk2 homolog CDKA;1 is a master regulator of meiosis needed for several aspects of meiosis such as chromosome synapsis. We identify the chromosome axis protein ASYNAPTIC 1 (ASY1), the Arabidopsis Hop1 homolog, which is required for synaptonemal complex formation, as a phospho-target of CDKA;1. Live cell imaging together with biochemical assays shows that CDK-dependent phosphorylation of ASY1 is required for its recruitment to the chromosome axis via ASYNAPTIC 3 (ASY3), the Arabidopsis Red1 homolog, counteracting the disassembly activity of the AAA+ ATPase PACHYTENE CHECKPOINT 2 (PCH2). Furthermore, we have mapped the closure motif of ASY1, typical for HORMA domain proteins, and provide evidence that the Cdk-dependent phosphorylation of ASY1 regulates the self-polymerization of ASY1 along the chromosome axis during early meiosis. Hence, the phosphorylation of ASY1 by CDKA;1 appears to be a two-pronged mechanism to initiate chromosome axis formation in meiosis.
HostingRepositoryPanoramaPublic
AnnounceDate2019-10-01
AnnouncementXMLSubmission_2019-10-01_08:39:32.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSara Stolze
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationListPhospho
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-10-10 09:56:05ID requested
12019-10-01 08:39:33announced
Publication List
Yang C, Sofroni K, Wijnker E, Hamamura Y, Carstens L, Harashima H, Stolze SC, Vezon D, Chelysheva L, Orban-Nemeth Z, Pochon G, Nakagami H, Schlögelhofer P, Grelon M, Schnittger A, The Arabidopsis Cdk1/Cdk2 homolog CDKA;1 controls chromosome axis assembly during plant meiosis. EMBO J, 39(3):e101625(2020) [pubmed]
Keyword List
submitter keyword: phosphorylation, PRM, Arabidopsis thaliana, meiosis
Contact List
Hirofumi Nakagami
contact affiliationProtein Mass Spectrometry, Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact emailnakagami@mpipz.mpg.de
lab head
Sara Stolze
contact affiliationProtein Mass Spectrometry, Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact emailstolze@mpipz.mpg.de
dataset submitter
Full Dataset Link List
Panorama Public dataset URI