PXD011334

PXD011334 is an original dataset announced via ProteomeXchange.

Title	Comprehensive analysis of the proteome, phosphoproteome and N-linked sialylated glycoproteome of C2C12 myoblasts
Description	C2C12 myoblast is a model that has been used extensively to study the process of skeletal muscle differentiation. Proteomics has advanced our understanding of skeletal muscle biology and the process of myogenesis. However, there is still no deep coverage of C2C12 myoblast proteome, which is important for the understanding of key drivers for the differentiation of skeletal muscle cells. Here, we conducted a multi-dimensional proteome profiling with TiSH strategy to get a comprehensive analysis of proteome, phosphoproteome and N-linked sialylated glycoproteome of C2C12 myoblasts. A total of 8313 protein groups were identified in C2C12 myoblasts, including 7827 protein groups from non-modified peptides, 3803 phosphoproteins and 977 formerly N-linked sialylated glycoproteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:15:50.546.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiulan Chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; complex glycosylation
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-10 07:49:54	ID requested
1	2020-11-27 08:05:05	announced
⏵ 2	2024-10-22 05:15:53	announced	2024-10-22: Updated project metadata.

Chen X, Sun Y, Zhang T, Roepstorff P, Yang F, Comprehensive Analysis of the Proteome and PTMomes of C2C12 Myoblasts Reveals that Sialylation Plays a Role in the Differentiation of Skeletal Muscle Cells. J Proteome Res, 20(1):222-235(2021) [pubmed]

10.1021/acs.jproteome.0c00353;

curator keyword: Biological

submitter keyword: PTMome,Mouse, skeletal myoblasts, proteome

Professor Fuquan Yang
contact affiliation	Key Laboratory of Protein and Peptide Pharmaceuticals & Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
contact email	fqyang@ibp.ac.cn
lab head
Xiulan Chen
contact affiliation	Institute of Biophysics
contact email	chenxiulan@moon.ibp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD011334

PRIDE project URI

• PRIDE
  1. PXD011334
     1. Label: PRIDE project
     2. Name: Comprehensive analysis of the proteome, phosphoproteome and N-linked sialylated glycoproteome of C2C12 myoblasts