PXD011322

PXD011322 is an original dataset announced via ProteomeXchange.

Title	KRAB Zincfinger Interactome - The interactome of KRAB zinc finger proteins reveals the evolutionary history of their functional diversification
Description	Krüppel-associated box (KRAB)-containing zinc finger proteins (KZFPs) represent the largest family of human transcription factors. The majority of its members bind to Transposable Elements (TEs), which they repress through the recruitment of the repressor KAP1 to their conserved KRAB domain. In addition to this recognized role, some KZFPs seem to favor different types of loci such as transcription start sites or display diverse functions such as imprinting and gene regulation. Intriguingly, a subset of KZFPs was shown not to recruit the corepressor KAP1. Therefore, we sought to get a better picture of KZFPs putative roles and their association with KAP1. In order to do so, we generated the interactomes of 101 KZFPs through Affinity Purification followed by Mass Spectrometry (AP-MS).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:21.791.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Heusel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-10 00:25:45	ID requested
1	2019-06-04 04:17:12	announced
2	2019-08-19 07:06:43	announced	Updated publication reference for PubMed record(s): 31403225.
⏵ 3	2024-10-22 04:49:22	announced	2024-10-22: Updated project metadata.

Helleboid PY, Heusel M, Duc J, Piot C, Thorball CW, Coluccio A, Pontis J, Imbeault M, Turelli P, Aebersold R, Trono D, The interactome of KRAB zinc finger proteins reveals the evolutionary history of their functional diversification. EMBO J, 38(18):e101220(2019) [pubmed]

10.15252/embj.2018101220;

curator keyword: Biological

submitter keyword: Zincfinger, AP-MS, Mass Spectrometry,KRAB

Rudolf Aebersold
contact affiliation	Institute of Molecular Systems Biology, ETH Zurich Faculty of Science, University of Zurich
contact email	aebersold@imsb.biol.ethz.ch
lab head
Moritz Heusel
contact affiliation	Lund University
contact email	moritz.heusel@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD011322

PRIDE project URI

• PRIDE
  1. PXD011322
     1. Label: PRIDE project
     2. Name: KRAB Zincfinger Interactome - The interactome of KRAB zinc finger proteins reveals the evolutionary history of their functional diversification