PXD011318

PXD011318 is an original dataset announced via ProteomeXchange.

Title	Chronic shisha exposure alters phosphoproteome of oral keratinocytes
Description	Shisha smoking has been epidemiologically linked to development of oral lesions as well as various cancers. However, few molecular studies investigate the pathobiology of shisha induced cancer. In this study we investigate the effects of chronic shisha exposure in an in vitro model using normal, immortalized, non-transformed oral keratinocytes. Quantitative proteomic and phosphoproteomic analyses were performed on OKF6/TERT1 cells exposed to shisha for a period of 8 months. Gene Ontology and pathway analysis of dysregulated proteins and phosphoproteins were carried out to identify significantly enriched biological processes and pathways in shisha exposed cells compared to parental cells. Chronic shisha exposure resulted in increased cell scattering phenomenon in OKF6/TERT1 cells. Quantitative data analysis revealed differential phosphorylation of 164 phosphopeptides (fold change ≥1.5, p≤0.05) corresponding to 136 proteins. Kinases such as PRKCD were seen to be hyperphosphorylated. Pathway analysis revealed an enrichment of hyperphosphorylated and/or overexpressed proteins involved in the mTORC1 and EIF4F complexes. These complexes are associated with initiation of protein translation and are known to the affected in cancers. Network analysis also highlighted a downregulation of proteins associated with Type I interferon signaling in shisha exposed cells. High throughput phosphoproteomic analysis revealed global perturbations to the molecular milieu of oral keratinocytes upon shisha exposure. Further studies are needed to validate putative targets in oral cancer patients with shisha smoking history.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:50:41.293.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aditi Chatterjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-09 06:09:23	ID requested
1	2019-07-23 08:24:04	announced
⏵ 2	2024-10-22 04:50:46	announced	2024-10-22: Updated project metadata.

10.1007/s12079-019-00528-4;

Patil S, Rajagopalan P, Patel K, Subbannayya T, Babu N, Mohan SV, Advani J, Sathe G, Bhandi S, Solanki HS, Sidransky D, Chatterjee A, Gowda H, Ferrari M, Chronic shisha exposure alters phosphoproteome of oral keratinocytes. J Cell Commun Signal, 13(3):281-289(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: oral cancer, hookah,Waterpipe tobacco smoking, narghile tobacco, carcinogenesis, high-throughput, OSCC, Orbitrap Fusion

Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, 7th floor, Discoverer building, International Tech Park, Bangalore 560 066
contact email	aditixchatterjee@gmail.com
lab head
Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India
contact email	aditixchatterjee@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011318

PRIDE project URI

• PRIDE
  1. PXD011318
     1. Label: PRIDE project
     2. Name: Chronic shisha exposure alters phosphoproteome of oral keratinocytes