PXD011314

PXD011314 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Profiling of Methylglyoxal Blood Metabolism and Advanced Glycation End-Product Proteome using a Chemical Probe
Description	Methylglyoxal (MG) is a toxic byproduct of the glycolytic pathway and is quantitatively the most important precursor to advanced glycation end-products (AGEs). Insight into which proteins and in particular their individual modification sites are central to understand the involvement of MG and AGE in diabetes and aging related diseases. Here, we present a method to simultaneously monitor protein AGE formation in biological samples by employing an alkyne-labeled methylglyoxal probe. We apply the method to blood and plasma to demonstrate the impact of blood cell glyoxalase activity on plasma protein AGE formation. We move on to isolate proteins modified by the MG probe and accordingly can present the first general inventory of more than 100 proteins and 300 binding sites of the methylglyoxal probe on plasma as well as erythrocytic proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:31.114.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johan Palmfeldt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-09 05:20:49	ID requested
1	2019-07-31 01:50:56	announced
⏵ 2	2024-10-22 04:49:39	announced	2024-10-22: Updated project metadata.

Publication List

10.1021/acschembio.8b00732;
Sibbersen C, Schou Oxvig AM, Bisgaard Olesen S, Nielsen CB, Galligan JJ, J, ø, rgensen KA, Palmfeldt J, Johannsen M, Profiling of Methylglyoxal Blood Metabolism and Advanced Glycation End-Product Proteome Using a Chemical Probe. ACS Chem Biol, 13(12):3294-3305(2018) [pubmed]

10.1021/acschembio.8b00732;

Sibbersen C, Schou Oxvig AM, Bisgaard Olesen S, Nielsen CB, Galligan JJ, J, ø, rgensen KA, Palmfeldt J, Johannsen M, Profiling of Methylglyoxal Blood Metabolism and Advanced Glycation End-Product Proteome Using a Chemical Probe. ACS Chem Biol, 13(12):3294-3305(2018) [pubmed]

Keyword List

curator keyword: Technical, Biological, Biomedical
submitter keyword: Human, erythrocytes, plasma, methylglyoxal

curator keyword: Technical, Biological, Biomedical

submitter keyword: Human, erythrocytes, plasma, methylglyoxal

Contact List

Johan Palmfeldt
contact affiliation	Aarhus University, Denmark
contact email	johan.palmfeldt@clin.au.dk
lab head
Johan Palmfeldt
contact affiliation	Aarhus University Hospital
contact email	johan.palmfeldt@clin.au.dk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011314
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011314

PRIDE project URI

Repository Record List

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