PXD011312

PXD011312 is an original dataset announced via ProteomeXchange.

Title	Mapping the proteome of the synaptic cleft through peroxidase-based reporter protein SynCAM1-HRP
Description	Synapses are the brain’s functional units connecting neurons into circuits that underlie memory and behavior. These specialized neuronal junctions are heterogenic in function and molecular composition, reflecting diverse health and disease states. Progress over the past years has shown that trans-synaptic adhesion molecules mediate synapse formation, specification, and differentiation during development. Among the prominently expressed synaptic cleft proteins are SynCAMs, a group of immunoglobulin molecules that engage in homo- and heterophilic interactions and that instruct synapse formation and guide synaptic maturation and that are specific for excitatory synapses. The current study describes the use of peroxidase-mediated proximity labeling to map the proteome of excitatory synapses using SynCAM1 as a reporter protein.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:24.834.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TuKiet Lam
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-09 02:07:44	ID requested
1	2018-12-04 08:40:23	announced
⏵ 2	2024-10-22 04:49:32	announced	2024-10-22: Updated project metadata.

Cijsouw T, Ramsey AM, Lam TT, Carbone BE, Blanpied TA, Biederer T, Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins. Proteomes, 6(4):(2018) [pubmed]

10.3390/proteomes6040048;

curator keyword: Biological

submitter keyword: LC MS/MS, synaptomics, synaptic cleft, synapse composition,SynCAM1, proximity labeling, Proteomics

TuKiet Lam
contact affiliation	Yale University, New Haven, CT 06511
contact email	tukiet.lam@yale.edu
lab head
TuKiet Lam
contact affiliation	Yale University
contact email	tukiet.lam@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD011312

PRIDE project URI

• PRIDE
  1. PXD011312
     1. Label: PRIDE project
     2. Name: Mapping the proteome of the synaptic cleft through peroxidase-based reporter protein SynCAM1-HRP