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PXD011312

PXD011312 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMapping the proteome of the synaptic cleft through peroxidase-based reporter protein SynCAM1-HRP
DescriptionSynapses are the brain’s functional units connecting neurons into circuits that underlie memory and behavior. These specialized neuronal junctions are heterogenic in function and molecular composition, reflecting diverse health and disease states. Progress over the past years has shown that trans-synaptic adhesion molecules mediate synapse formation, specification, and differentiation during development. Among the prominently expressed synaptic cleft proteins are SynCAMs, a group of immunoglobulin molecules that engage in homo- and heterophilic interactions and that instruct synapse formation and guide synaptic maturation and that are specific for excitatory synapses. The current study describes the use of peroxidase-mediated proximity labeling to map the proteome of excitatory synapses using SynCAM1 as a reporter protein.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:49:24.834.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTuKiet Lam
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-10-09 02:07:44ID requested
12018-12-04 08:40:23announced
22024-10-22 04:49:32announced2024-10-22: Updated project metadata.
Publication List
Cijsouw T, Ramsey AM, Lam TT, Carbone BE, Blanpied TA, Biederer T, Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins. Proteomes, 6(4):(2018) [pubmed]
10.3390/proteomes6040048;
Keyword List
curator keyword: Biological
submitter keyword: LC MS/MS, synaptomics, synaptic cleft, synapse composition,SynCAM1, proximity labeling, Proteomics
Contact List
TuKiet Lam
contact affiliationYale University, New Haven, CT 06511
contact emailtukiet.lam@yale.edu
lab head
TuKiet Lam
contact affiliationYale University
contact emailtukiet.lam@yale.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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