PXD011311

PXD011311 is an original dataset announced via ProteomeXchange.

Title	Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions
Description	The precise interplay between the mRNA codon and the tRNA anticodon is crucial for ensuring efficient and accurate translation by the ribosome. The insertion of RNA nucleobase derivatives in the mRNA allowed us to modulate the stability of the codon-anticodon interaction in the decoding site of bacterial and eukaryotic ribosomes, allowing an in-depth analysis of codon recognition. In addition to a quantitative analysis of the protein products that are formed in dependence of the modified codons, the interpretation of these RNA nucleobase derivatives by the ribosomal decoding site was also determined. For each modification, the translated peptides from the bacterial and eukaryotic systems were purified and analyzed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2018-11-21
AnnouncementXML	Submission_2018-11-21_10:09:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Hoernes
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	formylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-09 01:58:03	ID requested
⏵ 1	2018-11-21 10:09:52	announced

Hoernes TP, Faserl K, Juen MA, Kremser J, Gasser C, Fuchs E, Shi X, Siewert A, Lindner H, Kreutz C, Micura R, Joseph S, H, ö, bartner C, Westhof E, H, ü, ttenhofer A, Erlacher MD, Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions. Nat Commun, 9(1):4865(2018) [pubmed]

curator keyword: Biological

submitter keyword: biological, ribosome, decoding, translation elongation, codon-anticodon interactions, nucleotide modifications

Matthias David Erlacher
contact affiliation	Medical University of Innsbruck - Biocenter, Division of Genomics & RNomics
contact email	matthias.erlacher@i-med.ac.at
lab head
Thomas Hoernes
contact affiliation	Medical University of Innsbruck - Biocenter, Division of Genomics & RNomics
contact email	thomas.hoernes@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD011311

PRIDE project URI

• PRIDE
  1. PXD011311
     1. Label: PRIDE project
     2. Name: Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions