PXD011307 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Glycoproteomic analysis of MGL binding protein on acute T cell leukemia cells |
Description | O-glycosylation is generally initiated by the transfer of a N-acetylgalactosamine to Ser/Thr residues of proteins, forming the Tn antigen. This truncated surface glycan is expressed at high levels by tumor cells and is associated with higher metastatic behaviour and poor prognosis of patients. The Tn antigen is recognised by the C-type macrophage galactose lectin (MGL), which induces the activation of immunosuppressive responses. Here, we investigated the MGL binding proteins in Jurkat cells. The optimization of pull-down assays and subsequent glycoproteomic analysis by mass spectrometry, allowed us to identify 17 cell surface proteins as novel MGL-ligands. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:49:26.883.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yassene Mohammed |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | N-acetylhexosaminylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-08 07:50:08 | ID requested | |
1 | 2019-01-02 05:26:08 | announced | |
⏵ 2 | 2024-10-22 04:49:27 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.jproteome.8b00796; |
Pirro M, Schoof E, van Vliet SJ, Rombouts Y, Stella A, de Ru A, Mohammed Y, Wuhrer M, van Veelen PA, Hensbergen PJ, Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells. J Proteome Res, 18(3):1125-1132(2019) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Glycoproteomics, leukemia, O-glycosylation, Tn-antigen, lectin |
Contact List
Paul Hensbergen |
contact affiliation | Center for Proteomics and Metabolomics Leiden University Medical Center Tel +31-71-5266394 P.J.Hensbergen@lumc.nl https://www.lumc.nl/org/proteomics-metabolomics/ https://cpm.lumc.nl |
contact email | P.J.Hensbergen@lumc.nl |
lab head | |
Yassene Mohammed |
contact affiliation | LUMC/UVIC |
contact email | y.mohammed@lumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011307
- Label: PRIDE project
- Name: Glycoproteomic analysis of MGL binding protein on acute T cell leukemia cells