PXD011307

PXD011307 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Glycoproteomic analysis of MGL binding protein on acute T cell leukemia cells
Description	O-glycosylation is generally initiated by the transfer of a N-acetylgalactosamine to Ser/Thr residues of proteins, forming the Tn antigen. This truncated surface glycan is expressed at high levels by tumor cells and is associated with higher metastatic behaviour and poor prognosis of patients. The Tn antigen is recognised by the C-type macrophage galactose lectin (MGL), which induces the activation of immunosuppressive responses. Here, we investigated the MGL binding proteins in Jurkat cells. The optimization of pull-down assays and subsequent glycoproteomic analysis by mass spectrometry, allowed us to identify 17 cell surface proteins as novel MGL-ligands.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:26.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yassene Mohammed
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-acetylhexosaminylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-08 07:50:08	ID requested
1	2019-01-02 05:26:08	announced
⏵ 2	2024-10-22 04:49:27	announced	2024-10-22: Updated project metadata.

Publication List

10.1021/acs.jproteome.8b00796;
Pirro M, Schoof E, van Vliet SJ, Rombouts Y, Stella A, de Ru A, Mohammed Y, Wuhrer M, van Veelen PA, Hensbergen PJ, Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells. J Proteome Res, 18(3):1125-1132(2019) [pubmed]

10.1021/acs.jproteome.8b00796;

Pirro M, Schoof E, van Vliet SJ, Rombouts Y, Stella A, de Ru A, Mohammed Y, Wuhrer M, van Veelen PA, Hensbergen PJ, Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells. J Proteome Res, 18(3):1125-1132(2019) [pubmed]

Keyword List

curator keyword: Biological, Biomedical
submitter keyword: Glycoproteomics, leukemia, O-glycosylation, Tn-antigen, lectin

curator keyword: Biological, Biomedical

submitter keyword: Glycoproteomics, leukemia, O-glycosylation, Tn-antigen, lectin

Contact List

Paul Hensbergen
contact affiliation	Center for Proteomics and Metabolomics Leiden University Medical Center Tel +31-71-5266394 P.J.Hensbergen@lumc.nl https://www.lumc.nl/org/proteomics-metabolomics/ https://cpm.lumc.nl
contact email	P.J.Hensbergen@lumc.nl
lab head
Yassene Mohammed
contact affiliation	LUMC/UVIC
contact email	y.mohammed@lumc.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011307
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011307

PRIDE project URI

Repository Record List

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