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PXD011307

PXD011307 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlycoproteomic analysis of MGL binding protein on acute T cell leukemia cells
DescriptionO-glycosylation is generally initiated by the transfer of a N-acetylgalactosamine to Ser/Thr residues of proteins, forming the Tn antigen. This truncated surface glycan is expressed at high levels by tumor cells and is associated with higher metastatic behaviour and poor prognosis of patients. The Tn antigen is recognised by the C-type macrophage galactose lectin (MGL), which induces the activation of immunosuppressive responses. Here, we investigated the MGL binding proteins in Jurkat cells. The optimization of pull-down assays and subsequent glycoproteomic analysis by mass spectrometry, allowed us to identify 17 cell surface proteins as novel MGL-ligands.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:49:26.883.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYassene Mohammed
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListN-acetylhexosaminylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-10-08 07:50:08ID requested
12019-01-02 05:26:08announced
22024-10-22 04:49:27announced2024-10-22: Updated project metadata.
Publication List
10.1021/acs.jproteome.8b00796;
Pirro M, Schoof E, van Vliet SJ, Rombouts Y, Stella A, de Ru A, Mohammed Y, Wuhrer M, van Veelen PA, Hensbergen PJ, Glycoproteomic Analysis of MGL-Binding Proteins on Acute T-Cell Leukemia Cells. J Proteome Res, 18(3):1125-1132(2019) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Glycoproteomics, leukemia, O-glycosylation, Tn-antigen, lectin
Contact List
Paul Hensbergen
contact affiliationCenter for Proteomics and Metabolomics Leiden University Medical Center Tel +31-71-5266394 P.J.Hensbergen@lumc.nl https://www.lumc.nl/org/proteomics-metabolomics/ https://cpm.lumc.nl
contact emailP.J.Hensbergen@lumc.nl
lab head
Yassene Mohammed
contact affiliationLUMC/UVIC
contact emaily.mohammed@lumc.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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