PXD011306

PXD011306 is an original dataset announced via ProteomeXchange.

Title	Atta columbica seminal fluid and spermathecal fluid proteome
Description	Mature males and virgin queens from Atta colombica ants were collected in panama, and their seminal fluid (SF) and spermathecal fluid (SpF), respectively, were extracted and analysed by shot-gun proteomics. For this, using a pooled SF sample of 25 males from five colonies, squeezing their abdomens to collect 2-4ul of ejaculate per male from the extended endophallus. The SF supernatant was collected from the pooled ejaculate after 2 centrifugations for10 min at 13,000xg. The Spf was obtained form 4 different colonies collecting 10 virgin queens per colony and “artificially inseminating them with 10ul of Hayes. All 40 queens were allowed to recover for 12 hours before retrieving the content by dissecting and rupturing the spermathecal in Hayes. The samples were vortexed for 3 min, followed by centrifugation for 3 min at 13,500 xg to separate SpF from tissue.
HostingRepository	PRIDE
AnnounceDate	2019-01-03
AnnouncementXML	Submission_2019-01-03_02:21:37.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011306
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Julia Grassl
SpeciesList	scientific name: Atta columbica; NCBI TaxID: 592321;
ModificationList	Oxidation; Carbamidomethyl
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-08 04:03:00	ID requested
⏵ 1	2019-01-03 02:21:38	announced

Dosselli R, Grassl J, den Boer SPA, Kratz M, Moran JM, Boomsma JJ, Baer B, Protein-Level Interactions as Mediators of Sexual Conflict in Ants. Mol Cell Proteomics, 18(Suppl 1):S34-S45(2019) [pubmed]

submitter keyword: Atta colombica, Sexual conflict, DIGE, seminal fluid, spermathecal fluid.

Prof Boris Baer
contact affiliation	Centre for Integrative Bee Research (CIBER) Department of Entomology University of California Riverside, CA 92521United States of America
contact email	borisbar@ucr.edu
lab head
Julia Grassl
contact affiliation	Plant energy Biology Centre - School of Molecular Sciences UWA
contact email	julia.grassl@uwa.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011306
     1. Label: PRIDE project
     2. Name: Atta columbica seminal fluid and spermathecal fluid proteome