PXD011304

PXD011304 is an original dataset announced via ProteomeXchange.

Title	Macromolecular connectivity landscape of mammalian brain
Description	Connectivity webs mediate the unique biology of mammalian brain. Yet while cell and gene circuit maps are increasing in resolution, knowledge of the molecular interaction networks of the brain is limited. Here, we applied multidimensional biochemical fractionation with precision mass spectrometry to survey endogenous macromolecules in adult mouse brain. We defined a global ‘interactome’ landscape consisting of hundreds of multi-protein complexes, most never reported before. Brain selective assemblies exhibit distinctive biophysical and functional attributes, including enrichment for synaptic, RNA-binding and other evolutionarily conserved proteins showing tissue-, regional- and cell-type specificity. Strikingly, many macromolecules have links to diverse neurological disorders and disease variants, illustrating the broad pathophysiological relevance of the network. We validated a putative 15-member complex associated with Amyotrophic Lateral Sclerosis using reciprocal pulldowns and a transgenic rodent model, establishing balancing functions in alternative splicing and disease progression. This resource facilitates exploration of the mechanistic basis of neuronal function, synaptic plasticity and diseases of the central nervous system.
HostingRepository	PRIDE
AnnounceDate	2020-04-23
AnnouncementXML	Submission_2020-04-23_07:36:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sadhna Phanse
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-08 04:01:40	ID requested
⏵ 1	2020-04-23 07:36:49	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Mouse Brain, Bichemical fractionation, Synaptic and Neuronal proteins, Neurological diseases , Macromolecues, Multi-protein complexes, Protein-protein interactions

Dr. Andrew Emili
contact affiliation	Donnelly CCBR, University of Toronto, Toronto ON M5S 3E1, Canada
contact email	andrew.emili@utoronto.ca
lab head
Sadhna Phanse
contact affiliation	University of Toronto; University of Regina
contact email	srphanse@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD011304

PRIDE project URI

• PRIDE
  1. PXD011304
     1. Label: PRIDE project
     2. Name: Macromolecular connectivity landscape of mammalian brain