PXD011293

PXD011293 is an original dataset announced via ProteomeXchange.

Title	Chaperonin CCT Checkpoint Function in Basal Transcription Factor TFIID Assembly
Description	TFIID is a cornerstone of eukaryotic gene regulation. Distinct TFIID complexes with unique subunit composition exist and several TFIID subunits are shared with other complexes, conveying intricate cellular decision making to control subunit allocation and functional assembly of this essential transcription factor. However, the underlying molecular mechanisms remain poorly understood. Here, we used quantitative proteomics to examine TFIID submodules and assembly mechanisms in human cells. Structural and mutational analysis of the cytoplasmic TAF5/TAF6/TAF9 submodule identified novel interactions crucial for TFIID integrity, and for allocating TAF9 to TFIID or the SAGA co-activator complex. We discover a key checkpoint function for the chaperonin CCT, which specifically associates with nascent TAF5 for subsequent handover to TAF6/TAF9 and ultimate holo-TFIID formation. Our findings illustrate at the molecular level how multisubunit complexes are crafted in the cell, involving checkpoint decisions facilitated by a chaperone machine.
HostingRepository	PRIDE
AnnounceDate	2019-01-07
AnnouncementXML	Submission_2019-01-07_09:09:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harmjan Vos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-05 07:46:42	ID requested
⏵ 1	2019-01-07 09:09:54	announced

Antonova SV, Haffke M, Corradini E, Mikuciunas M, Low TY, Signor L, van Es RM, Gupta K, Scheer E, Vos HR, Tora L, Heck AJR, Timmers HTM, Berger I, Chaperonin CCT checkpoint function in basal transcription factor TFIID assembly. Nat Struct Mol Biol, 25(12):1119-1127(2018) [pubmed]

submitter keyword: TFIID, TRiC/CCT chaperonin, crystal structure, mutagenesis

H.T. Marc Timmers
contact affiliation	German Cancer Research Center (DKFZ), c/o Zentrale Klinische Forschung (ZKF), University Medical Center Freiburg, Breisacher Str. 66, 79106 Freiburg, Germany
contact email	m.timmers@dkfz-heidelberg.de
lab head
Harmjan Vos
contact affiliation	University Medical Center Utrecht Dept. Molecular Cancer Research
contact email	h.r.vos-3@umcutrecht.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011293

PRIDE project URI

• PRIDE
  1. PXD011293
     1. Label: PRIDE project
     2. Name: Chaperonin CCT Checkpoint Function in Basal Transcription Factor TFIID Assembly