PXD011292

PXD011292 is an original dataset announced via ProteomeXchange.

Title	Alteration of Proteomes in First-generation Cultures of Bacillus pumilus Spores Exposed to Outer Space
Description	Bacillus pumilus SAFR-032 was originally isolated from the Jet Propulsion Lab Spacecraft Assembly Facility and thoroughly characterized for its enhanced resistance to UV irradiation and oxidative stress. This unusual resistance of SAFR-032 is of particular concern in the context of planetary protection and calls for development of novel disinfection techniques to prevent extraterrestrial contamination. Previously, spores of SAFR-032 were exposed for 18 months to a variety of space conditions on board the International Space Station to investigate its resistance to Mars conditions and space travel. Here, proteomic characterization of vegetative SAFR-032 cells from space-surviving spores is presented in comparison to a ground control. Vegetative cells of the first passage were processed and subjected to quantitative proteomics using tandem mass tags. Approximately 60% of all proteins encoded by SAFR-032 were identified and 301 proteins were differentially expressed among the strains. The functional analysis of differentially expressed proteins revealed the downregulation of proteins related to carbohydrate transport/metabolism and energy production/conversion, which was validated by enzymatic assays. The same space-surviving strains showed upregulation of proteins related to competitive growth and stress response. Observed protein profiles provide insights into the possible molecular mechanisms of B. pumilus SAFR-032 to adapt and resist extreme extraterrestrial environments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:43:36.291.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Abby Chiang
SpeciesList	scientific name: Bacillus pumilus (strain SAFR-032); NCBI TaxID: 315750;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-05 07:32:12	ID requested
1	2019-05-21 00:26:39	announced
2	2019-06-17 03:19:22	announced	Updated publication reference for PubMed record(s): 31186338.
⏵ 3	2024-10-22 04:43:44	announced	2024-10-22: Updated project metadata.

Chiang AJ, Malli Mohan GB, Singh NK, Vaishampayan PA, Kalkum M, Venkateswaran K, Alteration of Proteomes in First-Generation Cultures of Bacillus pumilus Spores Exposed to Outer Space. mSystems, 4(4):(2019) [pubmed]

10.1128/msystems.00195-19;

submitter keyword: Space conditions, Tandem Mass Tag, Proteomics, UV resistance,Bacillus pumilus SAFR-032, Mars atmosphere

Markus Kalkum
contact affiliation	Beckman Research Institute of the City of Hope
contact email	mkalkum@coh.org
lab head
Abby Chiang
contact affiliation	City of Hope
contact email	jchiang@coh.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011292
     1. Label: PRIDE project
     2. Name: Alteration of Proteomes in First-generation Cultures of Bacillus pumilus Spores Exposed to Outer Space