PXD011290

PXD011290 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic and functional approaches reveal changes in sperm-specific proteins downstream KOR in human spermatozoa
Description	G-protein coupled receptors (GPCRs) belong to the seven transmembrane receptors superfamily that in response to external stimuli, transduce signals via G proteins to initiate different intracellular signaling pathways which culminate in specific cellular responses. Although in somatic cells the GPCR participate in a broad variety of physiological processes, the expression of diverse GPCRs at the plasma membrane of human spermatozoa suggests their involvement in the regulation of sperm fertility. Mature spermatozoa could present unique features in their molecular mechanisms downstream GPCRs due to the fact that they possess sperm-specific proteins and are transcriptionally and translationally silent. In order to decipher the signaling pathways engaged by this receptor superfamily in human spermatozoa, we selected the kappa-opioid receptor (KOR) as a study model and applied, for first time, phosphoproteomic approach based on TMT labeling and LC-MS/MS analyses combined with functional studies using a specific agonist of KOR, U50488H.
HostingRepository	PRIDE
AnnounceDate	2019-01-11
AnnouncementXML	Submission_2019-01-11_03:15:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Itziar Urizar Arenaza
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-05 05:50:05	ID requested
⏵ 1	2019-01-11 03:15:08	announced

Urizar-Arenaza I, Osinalde N, Akimov V, Puglia M, Candenas L, Pinto FM, Mu, ñ, oa-Hoyos I, Gianzo M, Matorras R, Irazusta J, Blagoev B, Subiran N, Kratchmarova I, Phosphoproteomic and Functional Analyses Reveal Sperm-specific Protein Changes Downstream of Kappa Opioid Receptor in Human Spermatozoa. Mol Cell Proteomics, 18(Suppl 1):S118-S131(2019) [pubmed]

curator keyword: Biological

submitter keyword: human spermatozoa, phosphoproteomics, KOR

Irina Kratchmarova
contact affiliation	Department of Biochemistry and Molecular Biology Campusvej 55 5230 Odense M Denmark ihk@bmb.sdu.dk Phone: +45 65502494
contact email	ihk@bmb.sdu.dk
lab head
Itziar Urizar Arenaza
contact affiliation	University of Basque Country (UPV/EHU)
contact email	itziar.urizara@ehu.eus
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011290

PRIDE project URI

• PRIDE
  1. PXD011290
     1. Label: PRIDE project
     2. Name: Phosphoproteomic and functional approaches reveal changes in sperm-specific proteins downstream KOR in human spermatozoa