PXD011286

PXD011286 is an original dataset announced via ProteomeXchange.

Title	Real-time visualization of drug resistance acquisition by horizontal gene transfer reveals a new role for AcrAB-TolC multidrug efflux pump
Description	In our study, we obtained evidence showing that, in the presence of tetracycline, AcrAB-TolC multidrug efflux pump was required for production of protein encoded by a conjugative plasmid acquired by bacterial conjugation. We then address the role of AcrAB-TolC multidrug efflux pump on the level of protein synthesis activity in the presence of tetracycline, which inhibits protein translation by targeting ribosomal 16S rRNA and competing with binding of the anticodon-stem loop of an A-site tRNA. We used a quantitative TMT-nanoLC-mass spectrometry approach to obtained the protein relative abundance ratio before and after incubation with tetracycline, and so in wild-type(MG1655, K12) and AcrAB-TolC mutant strains (ΔacrA, ΔacrB and ΔtolC).
HostingRepository	PRIDE
AnnounceDate	2019-07-16
AnnouncementXML	Submission_2019-07-16_06:55:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011286
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frederic DELOLME
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	TMT6plex; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-05 01:32:55	ID requested
⏵ 1	2019-07-16 06:55:04	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Antibiotic resistance acquisition by horizontal gene transfer AcrAB-TolC multidrug efflux Tc-induced inhibition of protein synthesis Escherichia coli TMT-nanoLC-MS

Frederic DELOLME / Christian LESTERLIN
contact affiliation	Protein Science Facility, SFR BioSciences CNRS UMS3444, Inserm US8, UCBL, ENS de Lyon, 50 Avenue Tony Garnier, 69007Lyon, France.
contact email	frederic.delolme@ibcp.fr
lab head
Frederic DELOLME
contact affiliation	CNRS UMS3444 Protein Science Facility
contact email	frederic.delolme@ibcp.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011286

PRIDE project URI

• PRIDE
  1. PXD011286
     1. Label: PRIDE project
     2. Name: Real-time visualization of drug resistance acquisition by horizontal gene transfer reveals a new role for AcrAB-TolC multidrug efflux pump