PXD011281

PXD011281 is an original dataset announced via ProteomeXchange.

Title	proteogenomic and metabolic adaptations of Staphylococcus aureus
Description	Background: Chronic lung infection of cystic fibrosis (CF) patients by Staphylococcus aureus is a well-established epidemiological fact. Indeed, S. aureus is the most commonly identified pathogen in the lungs of CF patients. Strikingly the molecular mechanisms underlying S. aureus persistency are not understood. Methods: To gain insights into S. aureus adaptation to CF lungs, we selected pairs of sequential S. aureus isolates from 3 patients with CF and from one patient with non-CF chronic lung disease. We used a combination of genomic, proteomic and metabolomic approaches with functional assays for in-depth characterization of S. aureus long-term persistence during chronic lung infection. Results: For the first time, we show that late S. aureus isolates from CF patients have an increased ability for intracellular survival in CFBE-F508del cells compared to ancestral early isolates. Importantly, the increased ability to persist intracellularly was confirmed for S. aureus isolates within the own patient F508del epithelial cells. An increased ability to form biofilm was also demonstrated. Furthermore, to explain observed phenotypic adaptations, we identified the underlying genetic modifications inducing altered protein expression profiles and notable metabolic changes. These modifications affect several metabolic pathways (e.g., pantothenate and fatty acids) and virulence regulators (encoded by agr and sae loci) that could constitute therapeutic targets. Conclusions: Our results strongly suggest that the intracellular environment might constitute an important niche of persistence and relapse necessitating adapted antibiotic treatments.
HostingRepository	PRIDE
AnnounceDate	2021-03-29
AnnouncementXML	Submission_2021-03-29_10:18:17.245.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-04 08:02:59	ID requested
⏵ 1	2021-03-29 10:18:17	announced

Dataset with its publication pending

submitter keyword: Staphylococcus aureus, proteogenomic

I Chiara GUERRERA
contact affiliation	Proteomic platform 3P5-Necker, Université Paris Descartes, SFR Necker, INSERM US24/ CNRS UMS3633 Paris
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011281
     1. Label: PRIDE project
     2. Name: proteogenomic and metabolic adaptations of Staphylococcus aureus