PXD011280

PXD011280 is an original dataset announced via ProteomeXchange.

Title	BioID proximity biotinylation-MS analysis of human main RAS GTPases in K-562 CML cells
Description	The human main RAS GTPases (KRAS4A, KRAS4B, NRAS and HRAS) represent major hubs of cellular signal transduction and are highly mutated in various different cancer entities. RAS GTPases are tightly controlled via different molecular mechanisms in order to coordinate their cellular signaling activity integrating extracellular and intracellular signals. In order to identify novel regulators of RAS signaling activity we have devised a BioID (BirA*-based proximity biotinylation)-Mass spectrometry-based approach in the human K-562 CML cell line. The deposited data set contains the acquired MS data after inducible expression, biotinylation, purification and proteomic analysis of KRAS4A, KRAS4B, NRAS and HRAS proteins. Further detailed information can be found in the appended sample data sheet and in the material and methods section of the manuscript Bigenzahn et al..
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2018-12-04_04:17:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011280
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Johannes Bigenzahn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Biotin; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-04 06:14:41	ID requested
1	2018-12-03 00:30:16	announced
⏵ 2	2018-12-04 04:17:40	announced	Updated project metadata.

Bigenzahn JW, Collu GM, Kartnig F, Pieraks M, Vladimer GI, Heinz LX, Sedlyarov V, Schischlik F, Fauster A, Rebsamen M, Parapatics K, Blomen VA, M, ü, ller AC, Winter GE, Kralovics R, Brummelkamp TR, Mlodzik M, Superti-Furga G, LZTR1 is a regulator of RAS ubiquitination and signaling. Science, 362(6419):1171-1177(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: CML, RAS GTPases, BioID, LC-MS

Giulio Superti-Furga
contact affiliation	CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Lazarettgasse 14, AKH BT 25.3 A-1090 Vienna, Austria
contact email	gsuperti@cemm.oeaw.ac.at
lab head
Johannes Bigenzahn
contact affiliation	CeMM, Vienna
contact email	jbigenzahn@cemm.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD011280

PRIDE project URI

• PRIDE
  1. PXD011280
     1. Label: PRIDE project
     2. Name: BioID proximity biotinylation-MS analysis of human main RAS GTPases in K-562 CML cells