PXD011279

PXD011279 is an original dataset announced via ProteomeXchange.

Title	Autophagy lipidation machinery regulates microtubule dynamics to maintain axonal homeostasis
Description	Axon integrity is crucial for neuronal function. To maintain axonal homeostasis neurons have evolved a unique organization of the microtubule (MT) cytoskeleton, which supports axonal morphology and provides tracks for intracellular transport. Although abnormal MT-based trafficking highlights the pathology of neurodegenerative diseases, the exact mechanism regulating MT dynamics in the axon remains enigmatic. Here we report on a novel regulation of MT dynamics by autophagy, the pathway, which previously has been linked to lysosomal degradation. We find that AuTophaGy(ATG)-related proteins required for LC3 lipid conjugation regulate MT stability via controlling the abundance of MT-binding protein CLASP2. This function of ATGs is independent of their role in protein degradation and requires the active zone protein ELKS1. Our results highlight a novel role of autophagy in neurons and suggest that pharmacological activation of autophagy may not only promote the degradation of bulk cytoplasmic material, but also impair axonal integrity via altering the MT stability.
HostingRepository	PRIDE
AnnounceDate	2020-03-25
AnnouncementXML	Submission_2020-04-22_04:26:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-04 05:36:14	ID requested
1	2020-03-25 07:24:19	announced
⏵ 2	2020-04-22 04:26:06	announced	2020-04-22: Updated publication reference for PubMed record(s): 32210230.

Negrete-Hurtado A, Overhoff M, Bera S, De Bruyckere E, Sch, ä, tzm, ü, ller K, Kye MJ, Qin C, Lammers M, Kondylis V, Neundorf I, Kononenko NL, Autophagy lipidation machinery regulates axonal microtubule dynamics but is dispensable for survival of mammalian neurons. Nat Commun, 11(1):1535(2020) [pubmed]

curator keyword: Biological

submitter keyword: autophagy, neuron, LC3, axonal homoeostasis, microtubule dynamics

Christian Frese
contact affiliation	CECAD Research Center, University of Cologne, Joseph-Stelzmann-Str. 26, 50931 Cologne, Germany
contact email	cfrese@uni-koeln.de
lab head
Christian Frese
contact affiliation	CECAD Research Center Cologne
contact email	cfrese@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD011279

PRIDE project URI

• PRIDE
  1. PXD011279
     1. Label: PRIDE project
     2. Name: Autophagy lipidation machinery regulates microtubule dynamics to maintain axonal homeostasis