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PXD011279 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAutophagy lipidation machinery regulates microtubule dynamics to maintain axonal homeostasis
DescriptionAxon integrity is crucial for neuronal function. To maintain axonal homeostasis neurons have evolved a unique organization of the microtubule (MT) cytoskeleton, which supports axonal morphology and provides tracks for intracellular transport. Although abnormal MT-based trafficking highlights the pathology of neurodegenerative diseases, the exact mechanism regulating MT dynamics in the axon remains enigmatic. Here we report on a novel regulation of MT dynamics by autophagy, the pathway, which previously has been linked to lysosomal degradation. We find that AuTophaGy(ATG)-related proteins required for LC3 lipid conjugation regulate MT stability via controlling the abundance of MT-binding protein CLASP2. This function of ATGs is independent of their role in protein degradation and requires the active zone protein ELKS1. Our results highlight a novel role of autophagy in neurons and suggest that pharmacological activation of autophagy may not only promote the degradation of bulk cytoplasmic material, but also impair axonal integrity via altering the MT stability.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Frese
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-10-04 05:36:14ID requested
12020-03-25 07:24:19announced
22020-04-22 04:26:06announced2020-04-22: Updated publication reference for PubMed record(s): 32210230.
Publication List
Negrete-Hurtado A, Overhoff M, Bera S, De Bruyckere E, Schätzmüller K, Kye MJ, Qin C, Lammers M, Kondylis V, Neundorf I, Kononenko NL, Autophagy lipidation machinery regulates axonal microtubule dynamics but is dispensable for survival of mammalian neurons. Nat Commun, 11(1):1535(2020) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: autophagy, neuron, LC3, axonal homoeostasis, microtubule dynamics
Contact List
Christian Frese
contact affiliationCECAD Research Center, University of Cologne, Joseph-Stelzmann-Str. 26, 50931 Cologne, Germany
contact emailcfrese@uni-koeln.de
lab head
Christian Frese
contact affiliationCECAD Research Center Cologne
contact emailcfrese@uni-koeln.de
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
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