PXD011262

PXD011262 is an original dataset announced via ProteomeXchange.

Title	Temporally & Spatially-Resolved Microproteomics and MALDI MSI Lipidomic Analysis for Traumatic Brain Injury
Description	Using an experimental TBI rat model of mild/moderate Controlled Cortical Impact (CCI) injury, we combined large-scale proteomics identification and relative quantification using Spatially-Resolved Microproteomics with MALDI MS Imaging of Lipids. Spatially by studying different regions in the brain post injury in a coronal view, with main focus on the injury site itself. Temporally by studying the acute and subacute phase post injury, including injured rat brains at 1 day, 3 days, 7 days, and 10 days post injury. Direct on-tissue micro-digestion followed by micoextraction from 1 mm2 surface area within the injured cortical tissue were subjected to LC-MS & MS/MS analysis using HR MS. In addition, several identified potential biomarkers within our study were used to stimulate dorsal root ganglion (DRG), astrocyte, and macrophage cell lines to obtain a better understanding of their role and contribution in the injury.
HostingRepository	PRIDE
AnnounceDate	2019-06-19
AnnouncementXML	Submission_2019-06-19_06:14:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jusal Quanico
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-03 08:02:13	ID requested
⏵ 1	2019-06-19 06:14:12	announced

Mallah K, Quanico J, Raffo-Romero A, Cardon T, Aboulouard S, Devos D, Kobeissy F, Zibara K, Salzet M, Fournier I, Mapping Spatiotemporal Microproteomics Landscape in Experimental Model of Traumatic Brain Injury Unveils a link to Parkinson's Disease. Mol Cell Proteomics, 18(8):1669-1682(2019) [pubmed]

submitter keyword: traumatic brain injury, spatiotemporal microproteomics, long-chain acylcarnitine, macrophage, astrocyte, substantia nigra

Michel Salzet
contact affiliation	PRISM Laboratory-INSERM U1192, University of Lille, France
contact email	michel.salzet@univ-lille.fr
lab head
Jusal Quanico
contact affiliation	PRISM- University of Lille 1
contact email	jusal.quanico@univ-lille1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD011262

PRIDE project URI

• PRIDE
  1. PXD011262
     1. Label: PRIDE project
     2. Name: Temporally & Spatially-Resolved Microproteomics and MALDI MSI Lipidomic Analysis for Traumatic Brain Injury