PXD011255

PXD011255 is an original dataset announced via ProteomeXchange.

Title	co-immunoprecipitation of the Arabidopsis G protein β subunit AGB1
Description	Heterotrimeric guanine nucleotide-binding (G) proteins are composed of Gα, Gβ, and Gγ subunits, and function as molecular switches in signal transduction. In Arabidopsis thaliana there are one canonical Gα (GPA1), three extra-large Gα (XLG1, XLG2 and XLG3), one Gβ (AGB1) and three Gγ (AGG1, AGG2 and AGG3) subunits. To elucidate AGB1 molecular signaling, we performed immunoprecipitation using plasma membrane enriched proteins followed by mass spectrometry to identify the protein interactors of AGB1. After eliminating proteins present in the control immunoprecipitation, commonly identified contaminants, and organellar proteins, a total of 103 candidate AGB1-associated proteins were confidently identified. We identified all of the G protein subunits except XLG1, receptor-like kinases (RLKs), Ca2+ signaling related proteins and 14-3-3-like proteins, all of which may couple with or modulate G protein signaling.
HostingRepository	PRIDE
AnnounceDate	2018-10-02
AnnouncementXML	Submission_2018-10-02_08:44:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011255
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yunqing Yu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Arg; HNE; GG; HCysThiolactone; Dethiomethyl; Hex(1)HexNAc(1)dHex(1); Acetylhypusine; Hex; Phosphopantetheine; Bromo; Lys->AminoadipicAcid; HexNAc(1)dHex(2); Decanoyl; Phospho; GlycerylPE; Hep; PhosphoHex; Deamidated; Hex(1)HexNAc(1); Acetyl; CHDH; Palmitoyl; HexNAc(1)dHex(1); Nitro; Cation:K; Delta:S(-1)Se(1); Octanoyl; HexNAc; Carbamidomethyl; Gln->pyro-Glu; cGMP+RMP-loss; Trioxidation; Glycerophospho; Glu->pyro-Glu; QQQTGG; QTGG; Carboxyethyl; Delta:H(2)C(2); Chlorination; Lys; Trimethyl; Ammonia-loss; phosphoRibosyl; Val->Arg; Methyl; Hex(1)HexNAc(2)dHex(2); Oxidation; Diphthamide; Arg-loss; Carboxy; Delta:H(4)C(2); Dicarbamidomethyl; Didehydroretinylidene; Quinone; Didehydro; Cation:Na; Trp->Kynurenin; Palmitoleyl; Sulfo; dHex(1)Hex(1); Hex(2)HexNAc(2); Iodo; HexN; 3-phosphoglyceryl; Formyl; Carbamyl; Arg->Orn; Lys->Allysine; DMPO; PhosphoribosyldephosphoCoA; Ala->Gln; PhosphoHexNAc; Tyr->Dha; Amino; dHex(1)Hex(2); Dehydrated; Dimethyl; Biotin; Dioxidation; Hex(2)
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-02 08:17:11	ID requested
⏵ 1	2018-10-02 08:44:07	announced

Yu Y, Chakravorty D, Assmann SM, -Subunit, AGB1, Interacts with FERONIA in RALF1-Regulated Stomatal Movement. Plant Physiol, 176(3):2426-2440(2018) [pubmed]

submitter keyword: heterotrimeric G protein, AGB1, co-immunoprecipitation, Arabidopsis thaliana

Sarah M. Assmann
contact affiliation	Biology Department Pennsylvania State University
contact email	sma3@psu.edu
lab head
Yunqing Yu
contact affiliation	Donald Danforth Plant Science Center
contact email	yunqingyu0605@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD011255

PRIDE project URI

• PRIDE
  1. PXD011255
     1. Label: PRIDE project
     2. Name: co-immunoprecipitation of the Arabidopsis G protein β subunit AGB1