PXD011250 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TFIIIC dinamically binds Alu elements to control gene expression through chromatin looping |
Description | Folding of the mammalian genome is governed by architectural proteins, such asCTCF. TFIIIC, a RNA polymerase III transcription factor, has been identified as aninsulator but its role in genome topology is totally unknown. Here, we show that TFIIICestablishes long-range genomic interactions that affect gene expression. Upon serumstarvation (SS), TFIIIC occupancy increases at Alu elements (AEs) near promoters ofcell cycle-related genes. Bound AEs become H3K18 hyper-acetylated and fold tocontact distal pre-loaded CTCF sites near other cell cycle genes. The promoters ofthese genes also become hyper-acetylated ensuring their basal transcription during SSand their increased expression during serum re-exposure. Ablation of TFIIIC ordeletion of the TFIIIC-bound AE that loops to the G2/M cycling F (CCNF) locus affectsits expression and nuclear positioning. These results illustrate a novel function ofhuman TFIIIC in changing 3D genome topology through the epigenetic state of AEs. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-26 |
AnnouncementXML | Submission_2020-05-26_12:22:54.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Roberto Ferrari |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-02 02:52:37 | ID requested | |
⏵ 1 | 2020-05-26 12:22:55 | announced | |
Publication List
Ferrari R, de Llobet Cucalon LI, Di Vona C, Le Dilly F, Vidal E, Lioutas A, Oliete JQ, Jochem L, Cutts E, Dieci G, Vannini A, Teichmann M, de la Luna S, Beato M, TFIIIC Binding to Alu Elements Controls Gene Expression via Chromatin Looping and Histone Acetylation. Mol Cell, 77(3):475-487.e11(2020) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human, breast cancer, TFIIIC, Alu |
Contact List
Miguel Beato |
contact affiliation | Gene Regulation, Stem Cells and Cancer Program Centre de Regulació Genòmica (CRG) The Barcelona Institute of Science and Technology PRBB Dr. Aiguader 88 E-08003 Barcelona Spain Tlf +34 93 316 0119 Fax +34 93 316 0099 e-mail: miguel.beato@crg.eu Secretary: Gemma Perez Tlf +34 93 316 0165 e-mail: gemma.perez@crg.eu www.crg.eu/4dgenome |
contact email | miguel.beato@crg.eu |
lab head | |
Roberto Ferrari |
contact affiliation | Center for Genomic Regulation |
contact email | roberto.ferrari@crg.eu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011250
- Label: PRIDE project
- Name: TFIIIC dinamically binds Alu elements to control gene expression through chromatin looping