PXD011244

PXD011244 is an original dataset announced via ProteomeXchange.

Title	Improved SILAC Method for Double Labeling of Bacterial Proteome
Description	Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with the advantages of reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quantification accuracy, limiting the application of SILAC in bacterial proteomics to auxotrophic bacteria or single labeling with lysine. In this study, we found that adding high concentrations of isotope-labeled (heavy) and natural (light) amino acids into SILAC minimal medium can efficiently inhibit the complicated amino acid conversions between each other. This simple and straightforward strategy facilitated the full incorporation of amino acids into the bacterial proteome with good accuracy. The high labeling efficiency can be reached in different bacteria by slightly modifying the supplement of amino acids in culture media, promoting the widespread application of SILAC technique in bacterial proteomics.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_02:32:55.856.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhao xinlu
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-01 08:28:40	ID requested
⏵ 1	2022-02-28 02:32:56	announced

Han J, Yi S, Zhao X, Zheng Y, Yang D, Du G, Yang XY, He QY, Sun X, Improved SILAC method for double labeling of bacterial proteome. J Proteomics, 194():89-98(2019) [pubmed]

curator keyword: Technical

submitter keyword: SILAC, bacteria, proteomics, double labeling

Xuesong Sun
contact affiliation	Key laboratory of functional protein research of guangdong higher education institutes, institute of life and health engineering, college of life science and technology, Jinan University, guangzhou 510632, China
contact email	tsunxs@jnu.edu.cn
lab head
zhao xinlu
contact affiliation	jinan university
contact email	xinlu-zhao@foxmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011244
     1. Label: PRIDE project
     2. Name: Improved SILAC Method for Double Labeling of Bacterial Proteome