PXD011218

PXD011218 is an original dataset announced via ProteomeXchange.

Title	Haloferax volcanii Proteome Response to Deletion of a Rhomboid Protease Gene
Description	Rhomboids are conserved intramembrane serine proteases involved in cell signaling processes. Their role in prokaryotes is scarcely known and remains to be investigated in Archaea. We previously constructed a rhomboid homologue deletion mutant (ΔrhoII) in Haloferax volcanii, which showed reduced motility, increased novobiocin sensitivity, and an Nglycosylation defect. To address the impact of rhoII deletion on H. volcanii physiology, the proteomes of mutant and parental strains were compared by shotgun proteomics. A total of 1847 proteins were identified (45.8% of H. volcanii predicted proteome), from which 103 differed in amount. Additionally, the mutant strain evidenced 99 proteins with altered electrophoretic migration, which suggested differential post-translational processing/modification. Integral membrane proteins that evidenced variations in concentration, electrophoretic migration, or semitryptic cleavage in the mutant were considered as potential RhoII targets. These included a PrsW protease homologue (which was less stable in the mutant strain), a predicted halocyanin, and six integral membrane proteins potentially related to the mutant glycosylation (S-layer glycoprotein, Agl15) and cell adhesion/motility (flagellin1, HVO_1153, PilA1, and PibD) defects. This study investigated for the first time the impact of a rhomboid protease on the whole proteome of an organism.
HostingRepository	PRIDE
AnnounceDate	2020-05-14
AnnouncementXML	Submission_2020-07-01_22:33:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Micaela Cerletti
SpeciesList	scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-28 05:56:25	ID requested
1	2020-05-14 03:49:32	announced
⏵ 2	2020-07-01 22:33:36	announced	2020-07-02: Updated publication reference for PubMed record(s): 32561711, 29301397.

Schulze S, Adams Z, Cerletti M, De Castro R, Ferreira-Cerca S, Fufezan C, Gim, é, nez MI, Hippler M, Jevtic Z, Kn, ü, ppel R, Legerme G, Lenz C, Marchfelder A, Maupin-Furlow J, Paggi RA, Pfeiffer F, Poetsch A, Urlaub H, Pohlschroder M, The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. Nat Commun, 11(1):3145(2020) [pubmed]

Costa MI, Cerletti M, Paggi RA, Tr, ö, tschel C, De Castro RE, Poetsch A, Gim, é, nez MI, Haloferax volcanii Proteome Response to Deletion of a Rhomboid Protease Gene. J Proteome Res, 17(3):961-977(2018) [pubmed]

curator keyword: Biological

submitter keyword: Archaea, Haloferax volcanii, rhomboid protease, shotgun proteomics, protease substrate

Rosana Esther De Castro
contact affiliation	Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata (UNMDP), CONICET, Mar del Plata (7600), Argentina
contact email	decastro@mdp.edu.ar
lab head
Micaela Cerletti
contact affiliation	Universidad Nacional de Mar del Plata
contact email	mcerletti@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011218
     1. Label: PRIDE project
     2. Name: Haloferax volcanii Proteome Response to Deletion of a Rhomboid Protease Gene