PXD011217

PXD011217 is an original dataset announced via ProteomeXchange.

Title	Proteomics of the Bee brain and in olfactory memory
Description	Previous work has shown that a series of honey bee brain proteins were probably linked to the proboscis extension reflex (PER) , a model studying insect behaviour. Herein, it was the aim of the study to generate a “synaptic proteome” and to investigate which proteins were paralleling olfactory conditioning. To address this information, we applied a non-sophisticated olfactory conditioning model using the proboscis extension reflex (PER) in the honeybee (Apis mellifera). Foraging honeybees were used in the study and three groups were formed, animals on water control, sucrose (PER) and olfactory conditioned (PER-conditioned) using 2-octanone. A synaptosomal preparation of honey bee brain for quantitative proteomics using stable isotope labelling (TMT 10plex) was performed. In addition a synaptosomal brain proteome was generated. Protein levels that were modified during olfactory conditioning were associated with “SNARE interactions in vesicular transport” (BET1 and VAMP7), ABC transporters, and fatty acid degradation pathways.. A honey bee synaptosomal proteome dataset including neurotransmitter receptors and transporters was generated.
HostingRepository	PRIDE
AnnounceDate	2019-05-27
AnnouncementXML	Submission_2019-05-27_04:08:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernando J. Sialana
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-28 05:52:46	ID requested
⏵ 1	2019-05-27 04:08:21	announced

Sialana FJ, Ribeiroda Silva Menegasso A, Smidak R, Hussein AM, Zavadil M, Rattei T, Lubec G, Sergio Palma M, Lubec J, Proteome Changes Paralleling the Olfactory Conditioning in the Forager Honey Bee and Provision of a Brain Proteomics Dataset. Proteomics, 19(13):e1900094(2019) [pubmed]

submitter keyword: bee, brain, olfactory memory, proboscis

Prof. Dr. Gert Lubec
contact affiliation	Paracelsus Private Medical University, Salzburg, Austria
contact email	gert.lubec@lubeclab.com
lab head
Fernando J. Sialana
contact affiliation	Pharmaceutical Chemistry, University of Vienna
contact email	fsialana@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD011217

PRIDE project URI

• PRIDE
  1. PXD011217
     1. Label: PRIDE project
     2. Name: Proteomics of the Bee brain and in olfactory memory