PXD011185

PXD011185 is an original dataset announced via ProteomeXchange.

Title	Proteomic dynamic changes in HDL subfractions from mouse plasma
Description	HDL proteome dynamics may determine HDL cardioprotective functions. We aimed to characterize proteome and lipid profiles in small, medium and large (S/M/L) HDL fractions and analyze their functions. We characterized mouse HDL fractions by their high phospholipid (PL) content and collected S/M/L-HDLsubfractions by using fast protein liquid chromatography. The pooled S/L/M-HDL elution was subjected to mass spectrometry (MS) analysis using a Qstar XL-MS system, performing HDL subpopulation proteomic analysis. Fifty-one HDL proteins (39 in S-HDL, 27 in M-HDL and 29 in L-HDL) were identified and grouped into 4 functional categories (5 in lipid metabolism, 24 in immune response, 7 in coagulation, and 14 others). There were 16, 3 and 7 proteins present in only the S-HDL, M-HDL and L-HDL subfractions, respectively, and 11 proteins overlapped in all S/M/L-HDL subfractions. The dynamic changes in relative HDL protein levels were then characterized based on their functional groups by biological and bioinformatical methods.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_14:52:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hang Xi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-25 01:40:35	ID requested
⏵ 1	2019-11-12 14:52:58	announced

Zhang Y, Gordon SM, Xi H, Choi S, Paz MA, Sun R, Yang W, Saredy J, Khan M, Remaley AT, Wang JF, Yang X, Wang H, HDL subclass proteomic analysis and functional implication of protein dynamic change during HDL maturation. Redox Biol, 24():101222(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Mouse, Plasam, HDL, HDL subfractions, ESI-MS/MS

Hong Wang
contact affiliation	Center for Metabolic Disease Research (CMDR) Lewis Katz School of Medicine at Temple University Medical Education and Research Building, Room 1060 3500 N. Broad Street Philadelphia, PA 19140, USA
contact email	hong.wang@temple.edu
lab head
Hang Xi
contact affiliation	Center for Metabolic Disease Research (CMDR) Lewis Katz School of Medicine at Temple University
contact email	hangxi@temple.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011185

PRIDE project URI

• PRIDE
  1. PXD011185
     1. Label: PRIDE project
     2. Name: Proteomic dynamic changes in HDL subfractions from mouse plasma