PXD011178

PXD011178 is an original dataset announced via ProteomeXchange.

Title	The use of LysargiNase complementary to trypsin in large scale phosphoproteome study for unambiguous phosphosite localization
Description	Understanding of kinase-guided signaling pathways requires identification and analysis of phosphorylation sites. Mass spectrometry (MS)-based phosphoproteomics is a rapid and highly sensitive approach for high-throughput identification of phosphorylation sites. However, the exact localization of phosphorylation sites which depends on trypsin-centric shotgun phosphoproteomics is often arbitrary and unreliable because of the limited product ion coverage in the MS2 spectra. Therefore, phosphorylation site determination from MS data with a single protease cannot be expected to be comprehensive, regardless of the strategy used for phosphopeptide detection. Here, we have explored the application of LysargiNase which was recently reported to mirror trypsin in specificity to cleave arginine and lysine residues exclusively at the N-terminal side. Our data demonstrates that the combined application of LysargiNase and trypsin results in higher sequence coverage with more complete ladder sequences which is helpful to pinpoint the precise phosphorylation sites. Therefore, the combined use of these two enzymes will partially overcome the limitations of trypsin-centric phosphoproteomics to achieve a more reliable phosphoproteome and to increase the identification of novel phosphorylation sites.
HostingRepository	PRIDE
AnnounceDate	2020-05-22
AnnouncementXML	Submission_2020-05-21_23:01:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011178
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Feng Xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-24 06:36:25	ID requested
⏵ 1	2020-05-21 23:01:39	announced

Xu F, Yu L, Peng X, Zhang J, Li S, Liu S, Yin Y, An Z, Wang F, Fu Y, Xu P, Unambiguous Phosphosite Localization through the Combination of Trypsin and LysargiNase Mirror Spectra in a Large-Scale Phosphoproteome Study. J Proteome Res, 19(6):2185-2194(2020) [pubmed]

submitter keyword: LysargiNase, phosphoproteome, phosphosite localization

Ping Xu
contact affiliation	State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Beijing Institute of Lifeomics, Beijing 102206, China
contact email	xuping_bprc@126.com
lab head
Feng Xu
contact affiliation	NCPSB
contact email	xf_future_2005@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011178
     1. Label: PRIDE project
     2. Name: The use of LysargiNase complementary to trypsin in large scale phosphoproteome study for unambiguous phosphosite localization