PXD011176

PXD011176 is an original dataset announced via ProteomeXchange.

Title	Cytological, biochemical and molecular events related to the embryogenic state in Douglas-fir
Description	Objectives: to characterize and to better understand differences at a protein level in embryonic and non-embryogenic tissues of embryonal masses in Douglas-fir. In Europe, Douglas-fir is a major species for reforestation with increasing demand for its wood. Harvested stems provide timber of outstanding wood quality, mechanical properties and durability. Commercial Douglas-fir plantations in France are limited by the ability to produce seed from the latest breeding developments. Somatic embryogenesis is considered a promising biotechnology for large-scale clonal propagation of forest trees, due to the high multiplication rates it can provide. Moreover, embryogenic cultures are amenable to both cryogenic storage for long-term preservation of genetic resources and genetic engineering (including genome editing) for functional characterization of genes expressed during embryogenesis. In conifers, embryogenic cultures take the form of embryonal mass made up of early differentiated cells forming immature somatic embryos that proliferate via cleavage polyembryony. In Douglas-fir embryogenic lines consisting in embryonal mass have been compared to non-embryogenic callus during their proliferation. Comparison of proteomes (free-gel proteomics) of embryonal mass vs non-embryogenic callus were performed.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:14:25.711.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephane Claverol
SpeciesList	scientific name: Pseudotsuga menziesii; NCBI TaxID: 3357;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-24 06:31:52	ID requested
1	2019-05-16 23:46:54	announced
⏵ 2	2024-10-22 04:14:28	announced	2024-10-22: Updated project metadata.

Gautier F, Label P, Eli, á, š, ov, á K, Lepl, é JC, Motyka V, Boizot N, Vondr, á, kov, á Z, Malbeck J, Tr, á, vn, í, č, kov, á A, Le Mett, é C, Lesage-Descauses MC, Lomenech AM, Trontin JF, Costa G, Lelu-Walter MA, Teyssier C, [Mirb.]). Front Plant Sci, 10():118(2019) [pubmed]

10.3389/fpls.2019.00118;

submitter keyword: phytohormone,Pseudostuga, non-embryogenic callus, transcriptomic, soluble carbohydrate, embryonal mass, proteomic, histo-cytology

Stephane Claverol
contact affiliation	Plateforme Proteome, Centre Genomique Fonctionnelle Bordeaux, Bordeaux, France
contact email	stephane.claverol@u-bordeaux.fr
lab head
Stephane Claverol
contact affiliation	Plateforme Proteome
contact email	stephane.claverol@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD011176

PRIDE project URI

• PRIDE
  1. PXD011176
     1. Label: PRIDE project
     2. Name: Cytological, biochemical and molecular events related to the embryogenic state in Douglas-fir