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PXD011176

PXD011176 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCytological, biochemical and molecular events related to the embryogenic state in Douglas-fir
DescriptionObjectives: to characterize and to better understand differences at a protein level in embryonic and non-embryogenic tissues of embryonal masses in Douglas-fir. In Europe, Douglas-fir is a major species for reforestation with increasing demand for its wood. Harvested stems provide timber of outstanding wood quality, mechanical properties and durability. Commercial Douglas-fir plantations in France are limited by the ability to produce seed from the latest breeding developments. Somatic embryogenesis is considered a promising biotechnology for large-scale clonal propagation of forest trees, due to the high multiplication rates it can provide. Moreover, embryogenic cultures are amenable to both cryogenic storage for long-term preservation of genetic resources and genetic engineering (including genome editing) for functional characterization of genes expressed during embryogenesis. In conifers, embryogenic cultures take the form of embryonal mass made up of early differentiated cells forming immature somatic embryos that proliferate via cleavage polyembryony. In Douglas-fir embryogenic lines consisting in embryonal mass have been compared to non-embryogenic callus during their proliferation. Comparison of proteomes (free-gel proteomics) of embryonal mass vs non-embryogenic callus were performed.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:14:25.711.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephane Claverol
SpeciesList scientific name: Pseudotsuga menziesii; NCBI TaxID: 3357;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-24 06:31:52ID requested
12019-05-16 23:46:54announced
22024-10-22 04:14:28announced2024-10-22: Updated project metadata.
Publication List
Gautier F, Label P, Eli, á, š, ov, á K, Lepl, é JC, Motyka V, Boizot N, Vondr, á, kov, á Z, Malbeck J, Tr, á, vn, í, č, kov, á A, Le Mett, é C, Lesage-Descauses MC, Lomenech AM, Trontin JF, Costa G, Lelu-Walter MA, Teyssier C, [Mirb.]). Front Plant Sci, 10():118(2019) [pubmed]
10.3389/fpls.2019.00118;
Keyword List
submitter keyword: phytohormone,Pseudostuga, non-embryogenic callus, transcriptomic, soluble carbohydrate, embryonal mass, proteomic, histo-cytology
Contact List
Stephane Claverol
contact affiliationPlateforme Proteome, Centre Genomique Fonctionnelle Bordeaux, Bordeaux, France
contact emailstephane.claverol@u-bordeaux.fr
lab head
Stephane Claverol
contact affiliationPlateforme Proteome
contact emailstephane.claverol@u-bordeaux.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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