PXD011173

PXD011173 is an original dataset announced via ProteomeXchange.

Title	Human FZD6 phosphorylation LC-MS/MS
Description	Frizzleds (FZD) function as receptors for secreted lipoglycoproteins of the Wingless/Int-1 (WNT) family, initiating an important signal transduction network in multicellular organisms. FZDs are classified as G protein-coupled receptors (GPCRs), which control a wide variety of physiological functions. GPCRs are well known to be regulated by phosphorylation, which can lead to specification of downstream signaling or receptor desensitization. The role and underlying mechanisms of phosphorylation of FZDs remains largely unexplored. Here, we investigated the phosphorylation of human FZD6. In order to assess FZD6 phosphorylation experimentally, we employed mass spectrometry. HEK293 cells expressing FZD6-GFP were treated with either control, WNT-3A or WNT-5A containing conditioned medium for 30 min and processed for LC-MS/MS analysis. Six phosphorylated serine residues were detected in the C-terminus of FZD6 − S592, S620, S629, S648, S653 and S656.
HostingRepository	PRIDE
AnnounceDate	2018-10-18
AnnouncementXML	Submission_2018-10-18_01:43:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Potesil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-24 05:47:26	ID requested
⏵ 1	2018-10-18 01:43:42	announced

Strakova K, Kowalski-Jahn M, Gybel T, Valnohova J, Dhople VM, Harnos J, Bernatik O, Ganji RS, Zdrahal Z, Mulder J, Lindskog C, Bryja V, Schulte G, Dishevelled enables casein kinase 1-mediated phosphorylation of Frizzled 6 required for cell membrane localization. J Biol Chem, 293(48):18477-18493(2018) [pubmed]

curator keyword: Biological

submitter keyword: Frizzled, FZD6, phosphorylation, WNT, WNT-3A, WNT-5A, LC-MS/MS, titanuim dioxide, label-free

Vishnu M Dhople
contact affiliation	Universitätsmedizin Greifswald Interfakultäres Institut für Genetik und Funktionelle Genomforschung Abteilung für Funktionelle Genomforschung Felix-Hausdorff-Straße 8 17487 Greifswald Germany
contact email	dhoplevm@uni-greifswald.de
lab head
David Potesil
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	david.potesil@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD011173

PRIDE project URI

• PRIDE
  1. PXD011173
     1. Label: PRIDE project
     2. Name: Human FZD6 phosphorylation LC-MS/MS