PXD011168

PXD011168 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A nanoLC-MS/MS system using a nonporous reverse phase column for ultrasensitive proteome analysis
Description	The design of proteome projects is often restricted by the available amounts of sample, and thus there has been much effort to date to improve the sensitivity of proteome analyses. We have developed a new ultrasensitive nanoLC-MS system for the proteome analysis of microscale samples using a nonporous reverse phase column. Although nonporous particles have low binding capacity due to low functional group density, we found that the use of C30 particles compensated for this disadvantage and nanoLC-MS proteome analyses of microscale samples using a C30-packed column exhibited superior performance compared to porous C18 columns.
HostingRepository	jPOST
AnnounceDate	2018-09-24
AnnouncementXML	Submission_2022-09-18_03:59:22.904.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yusuke Kawashima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-24 02:26:33	ID requested
1	2018-09-24 08:00:04	announced
2	2018-10-26 22:41:05	announced	Updated PubMed.
3	2021-02-04 16:43:39	announced	2021-02-04: Updated FTP location.
⏵ 4	2022-09-18 03:59:23	announced	2022-09-18: Updated FTP location.

Publication List

Kawashima Y, Ohara O, Development of a NanoLC-MS/MS System Using a Nonporous Reverse Phase Column for Ultrasensitive Proteome Analysis. Anal Chem, 90(21):12334-12338(2018) [pubmed]

Kawashima Y, Ohara O, Development of a NanoLC-MS/MS System Using a Nonporous Reverse Phase Column for Ultrasensitive Proteome Analysis. Anal Chem, 90(21):12334-12338(2018) [pubmed]

Keyword List

submitter keyword: nonporous C30 column, nonporous particle, ultrasensitive proteomics, HEK293

submitter keyword: nonporous C30 column, nonporous particle, ultrasensitive proteomics, HEK293

Contact List

Yusuke Kawashima
lab head
Yusuke Kawashima
contact affiliation	Kazusa DNA Research Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000499/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000499/