PXD011167

PXD011167 is an original dataset announced via ProteomeXchange.

Title	H89 Phosphoproteomics in PKA-Null Cells
Description	Protein phosphorylation plays a crucial role in propagating cellular responses to both internal and external cues. One of the most important kinases responsible for protein phosphorylation is protein kinase A (PKA). N-[2-p-bromocinnamylamino-ethyl]-5-isoquinolinesulphonamide (H89) is often used as a “PKA specific inhibitor” to study the involvement of PKA in signaling pathways. However, evidence from cell-free experiments suggested that H89 can also inhibit other kinases. In this experiment, previously-generated mouse collecting duct cell lines with and without PKA were treated with H89, followed by mass spectrometry-based phosphoproteomics to globally assess changes in phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2019-03-28
AnnouncementXML	Submission_2019-03-28_01:42:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kavee Limbutara
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-24 02:09:32	ID requested
⏵ 1	2019-03-28 01:42:46	announced

Limbutara K, Kelleher A, Yang CR, Raghuram V, Knepper MA, Phosphorylation Changes in Response to Kinase Inhibitor H89 in PKA-Null Cells. Sci Rep, 9(1):2814(2019) [pubmed]

curator keyword: Biological

submitter keyword: H89, PKA, kinase inhibitor, phosphoproteomics

Mark A. Knepper
contact affiliation	Epithelial Systems Biology Laboratory, Systems Biology Center, NHLBI, NIH
contact email	knepperm@nhlbi.nih.gov
lab head
Kavee Limbutara
contact affiliation	Epithelial Systems Biology Laboratory, Systems Biology Center, NHLBI, NIH
contact email	kavee.limbutara@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011167

PRIDE project URI

• PRIDE
  1. PXD011167
     1. Label: PRIDE project
     2. Name: H89 Phosphoproteomics in PKA-Null Cells