PXD011166

PXD011166 is an original dataset announced via ProteomeXchange.

Title	Interspecies and intersubstrate comparison of the biomass-degrading capacity of five filamentous fungi
Description	The efficiency of microorganisms to degrade lignified plants is of great importance in Earth’s carbon cycle, but also in industrial biorefinery processes, such as for biofuel production. Here, we present a large-scale proteomics approach to investigate and compare the enzymatic response of five filamentous fungi when grown on five very different substrates: bagasse, birch, spruce, cellulose and glucose. The five fungi included the ascomycetes Aspergillus terreus, Hypocrea jecorina, Myceliophthora thermophila, Neurospora crassa and the white-rot basidiomycete Phanerochaete chrysosporium, all expressing a diverse repertoire of enzymes. Many studies have previously been performed with these fungi under various growth conditions, but this study is unique as it presents comparable quantitative protein abundance values across five filamentous fungi and five diverse substrates that allows for direct comparison of fungal response to the different substrates; this approach gives indications to substrate specificity of individual carbohydrate-active enzymes (CAZymes) and identifies several novel co-expressed non-CAZymes. Specifically, we present a quantitative comparison of 34 lytic polysaccharide monooxidenases (LPMOs), which are crucial enzymes in biomass deconstruction.
HostingRepository	PRIDE
AnnounceDate	2020-11-25
AnnouncementXML	Submission_2020-11-24_22:48:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Neurospora crassa; NCBI TaxID: 5141; scientific name: Thermothelomyces thermophila ATCC 42464; NCBI TaxID: 573729; scientific name: Aspergillus terreus NIH2624; NCBI TaxID: 341663; scientific name: Hypocrea jecorina (strain QM6a) (Trichoderma reesei); NCBI TaxID: 431241; scientific name: Phanerochaete chrysosporium; NCBI TaxID: 5306;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-24 01:54:33	ID requested
⏵ 1	2020-11-24 22:48:57	announced

Arntzen M Ø, Bengtsson O, V, á, rnai A, Delogu F, Mathiesen G, Eijsink VGH, Quantitative comparison of the biomass-degrading enzyme repertoires of five filamentous fungi. Sci Rep, 10(1):20267(2020) [pubmed]

curator keyword: Metaproteomics, Biological

submitter keyword: proteomics, polysaccharide degradation, Aspergillus terreus, Hypocrea jecorina, Trichoderma reesei, Myceliophthora thermophila, Neurospora crassa, Phanerochaete chrysosporium

Vincent Eijsink
contact affiliation	Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences Post Office Box 5003 1432, Ås Norway
contact email	vincent.eijsink@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD011166

PRIDE project URI

• PRIDE
  1. PXD011166
     1. Label: PRIDE project
     2. Name: Interspecies and intersubstrate comparison of the biomass-degrading capacity of five filamentous fungi