PXD011157

PXD011157 is an original dataset announced via ProteomeXchange.

Title	Virulence factors produced by Staphylococcus aureus biofilms have a moonlighting function contributing to biofilm integrity
Description	We comprehensively profiled intracellular and ECM proteomes of S. aureus flow biofilms and complemented these data by metabolic footprint analysis and phenotypic assays. We show that S. aureus biofilms produce high amounts of secreted, moonlighting virulence factors stabilizing the ECM. Mechanistically, we propose that these alkaline virulence factors get protonated in an acidified ECM (due to the release of acids upon fermentation) mediating electrostatic interactions with anionic cell surface components and metabolites, which leads to cell aggregation and ECM stabilization.
HostingRepository	PRIDE
AnnounceDate	2019-03-13
AnnouncementXML	Submission_2019-03-12_21:09:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Staphylococcus aureus subsp. aureus NCTC 8325; NCBI TaxID: 93061;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-21 05:48:01	ID requested
⏵ 1	2019-03-12 21:09:32	announced

Graf AC, Leonard A, Sch, ä, uble M, Rieckmann LM, Hoyer J, Maass S, Lalk M, Becher D, Pan, é, -Farr, é J, Riedel K, Biofilms Have a Moonlighting Function Contributing to Biofilm Integrity. Mol Cell Proteomics, 18(6):1036-1053(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Staphylococcus aureus, Biofilm, ECM

Riedel, Katharina
contact affiliation	University of Greifswald; Institute of Microbiology; Dep. of Microbial Physiology and Molecular Biology
contact email	riedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	Ernst Moritz Arndt University Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011157

PRIDE project URI

• PRIDE
  1. PXD011157
     1. Label: PRIDE project
     2. Name: Virulence factors produced by Staphylococcus aureus biofilms have a moonlighting function contributing to biofilm integrity