PXD011157 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Virulence factors produced by Staphylococcus aureus biofilms have a moonlighting function contributing to biofilm integrity |
Description | We comprehensively profiled intracellular and ECM proteomes of S. aureus flow biofilms and complemented these data by metabolic footprint analysis and phenotypic assays. We show that S. aureus biofilms produce high amounts of secreted, moonlighting virulence factors stabilizing the ECM. Mechanistically, we propose that these alkaline virulence factors get protonated in an acidified ECM (due to the release of acids upon fermentation) mediating electrostatic interactions with anionic cell surface components and metabolites, which leads to cell aggregation and ECM stabilization. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:15:56.446.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Daniela Zuehlke |
SpeciesList | scientific name: Staphylococcus aureus subsp. aureus NCTC 8325; NCBI TaxID: 93061; |
ModificationList | monohydroxylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-21 05:48:01 | ID requested | |
1 | 2019-03-12 21:09:32 | announced | |
⏵ 2 | 2024-10-22 04:15:57 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra118.001120; |
Graf AC, Leonard A, Sch, ä, uble M, Rieckmann LM, Hoyer J, Maass S, Lalk M, Becher D, Pan, é, -Farr, é J, Riedel K, Biofilms Have a Moonlighting Function Contributing to Biofilm Integrity. Mol Cell Proteomics, 18(6):1036-1053(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: ECM, Biofilm,Staphylococcus aureus |
Contact List
Riedel, Katharina |
contact affiliation | University of Greifswald; Institute of Microbiology; Dep. of Microbial Physiology and Molecular Biology |
contact email | riedela@uni-greifswald.de |
lab head | |
Daniela Zuehlke |
contact affiliation | University of Greifswald, Institute of Microbiology |
contact email | zuehlke@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011157 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011157
- Label: PRIDE project
- Name: Virulence factors produced by Staphylococcus aureus biofilms have a moonlighting function contributing to biofilm integrity