PXD011149

PXD011149 is an original dataset announced via ProteomeXchange.

Title	Honeybee antennae LC-MSMS - The timing and amplitude of reproductive effort are central life history variables for all organisms
Description	The timing and amplitude of reproductive effort are central life history variables for all organisms. In social insects, reproductive effort is collectively controlled at the colony level but little is known about the mechanisms that determine how much colonies invest in reproduction. As part of their female reproductive investment, honey bee colonies raise multiple new queens by feeding royal jelly to female larvae. Artificial selection for commercial royal jelly production in China has generated over the past 40 years a stock of royal jelly bees that raises an order of magnitude more queens and provisions each queen with >3x more royal jelly than unselected stock. Here we establish in a reciprocal cross-fostering experiment that this dramatic shift in social phenotype is due to changes in the nurse bees that care for the brood. We demonstrate higher electrophysiological responsiveness to brood pheromones in royal jelly bees than in unselected bees. Comparing the antennal proteome of unselected and royal jelly bees, we identify proteins involved in chemosensation and energy metabolism as candidates for the observed differences. We confirm several candidates, most prominently OBP16 and CSP4, with quantitative differences of corresponding mRNA levels and functional binding assays between the brood pheromones and the chemosensory proteins. Furthermore, we complement analyses of brood volatiles and electrophysiological recordings with behavioral attraction assays to confirm the presumed biological function of one newly discovered and two existing larval pheromones. Together, these findings help our understanding of pheromonal communication in honey bees and explain how sensory changes in nurse honey bees as alloparental caregivers have evolved in response to artificial selection, leading to a profound shift in colony-level resource allocation to sexual reproduction.
HostingRepository	PRIDE
AnnounceDate	2020-03-12
AnnouncementXML	Submission_2020-03-12_03:45:59.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011149
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fan Wu
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-20 01:13:02	ID requested
1	2019-08-01 07:20:11	announced
⏵ 2	2020-03-12 03:46:00	announced	2020-03-12: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: honeybee, antennae

Jianke Li
contact affiliation	Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Chinese Academy of Agricultural Science, Beijing, China;
contact email	apislijk@126.com
lab head
Fan Wu
contact affiliation	Institute of Apisculture research, CAAS
contact email	wufan2102@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD011149

PRIDE project URI

• PRIDE
  1. PXD011149
     1. Label: PRIDE project
     2. Name: Honeybee antennae LC-MSMS - The timing and amplitude of reproductive effort are central life history variables for all organisms