PXD011146 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification and functional annotation of the Src-regulated kinome, part 2 |
Description | Despite significant progress, our understanding of how specific oncogenes transform cells is still limited and likely underestimates the complexity of downstream signalling events. Herein, we describe a novel, integrated approach that addresses this knowledge gap. This utilizes mass spectrometry-based chemical proteomics to characterize the global impact of an oncogene on the expressed kinome, and then functionally annotates the regulated kinases. As an example, we identified approximately one hundred protein kinases exhibiting altered expression and/or phosphorylation in Src-transformed mammary epithelial cells. Screening with corresponding siRNAs identified nine kinases, including SGK1, as being essential for Src-induced transformation. In contrast, MAP4K5 suppressed transformation in a manner enhanced by S335 phosphorylation. In triple negative breast cancer cells, Src positively regulated SGK1 expression and combined inhibition of Src and SGK1 was more effective at inhibiting cell proliferation than either treatment alone. Therefore, this approach not only provides major mechanistic insights into oncogenic transformation but also aids the design of improved therapeutic strategies. |
HostingRepository | PRIDE |
AnnounceDate | 2018-11-21 |
AnnouncementXML | Submission_2018-11-21_10:00:54.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hugh Ma |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-19 05:40:14 | ID requested | |
⏵ 1 | 2018-11-21 10:00:55 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Kinome, LC-MSMS, phosphoproteome |
Contact List
Roger Daly |
contact affiliation | Monash University |
contact email | roger.daly@monash.edu |
lab head | |
Hugh Ma |
contact affiliation | Monash Uni |
contact email | hugh.ma@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011146
- Label: PRIDE project
- Name: Identification and functional annotation of the Src-regulated kinome, part 2