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PXD011146

PXD011146 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification and functional annotation of the Src-regulated kinome, part 2
DescriptionDespite significant progress, our understanding of how specific oncogenes transform cells is still limited and likely underestimates the complexity of downstream signalling events. Herein, we describe a novel, integrated approach that addresses this knowledge gap. This utilizes mass spectrometry-based chemical proteomics to characterize the global impact of an oncogene on the expressed kinome, and then functionally annotates the regulated kinases. As an example, we identified approximately one hundred protein kinases exhibiting altered expression and/or phosphorylation in Src-transformed mammary epithelial cells. Screening with corresponding siRNAs identified nine kinases, including SGK1, as being essential for Src-induced transformation. In contrast, MAP4K5 suppressed transformation in a manner enhanced by S335 phosphorylation. In triple negative breast cancer cells, Src positively regulated SGK1 expression and combined inhibition of Src and SGK1 was more effective at inhibiting cell proliferation than either treatment alone. Therefore, this approach not only provides major mechanistic insights into oncogenic transformation but also aids the design of improved therapeutic strategies.
HostingRepositoryPRIDE
AnnounceDate2018-11-21
AnnouncementXMLSubmission_2018-11-21_10:00:54.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHugh Ma
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-19 05:40:14ID requested
12018-11-21 10:00:55announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Kinome, LC-MSMS, phosphoproteome
Contact List
Roger Daly
contact affiliationMonash University
contact emailroger.daly@monash.edu
lab head
Hugh Ma
contact affiliationMonash Uni
contact emailhugh.ma@monash.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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