PXD011131

PXD011131 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide detection of cysteine nitrosylation targets and motifs using bio-orthogonal cleavable linker based enrichment and switch technique (Cys-BOOST)
Description	Cysteine (Cys) reversible post-translational modifications (PTMs) are emerging as important players in cellular signaling and redox homeostasis. Here we present Cys-BOOST a novel strategy for LC-MS/MS based quantitative analysis of reversibly modified Cys using switch technique, enrichment via bio-orthogonal cleavable linker and quantification using tandem mas tag (TMT) reagents. We performed direct comparison of Cys-BOOST (n=3) with iodo-TMT (n=3) by analyzing the total CysCys from HeLa cell extracts. As a result higher sensitivity (25,019 vs 9,966 Cys peptides), specificity (98 vs 74 %) and technical reproducibility were obtained by Cys-BOOST. In addition, the application of Cys-BOOST for the analysis of Cys nitrosylation (SNO) in S-nitrosoglutathione (GSNO) treated and non-treated HeLa cell extracts lead to the identification of unprecedented number of SNO proteins (3,537), SNO peptides (9,314) and unique SNO sites (8,304). Based on the quantitative data we describe SNO consensus motifs for endogenous SNO and SNO sites with differential reactivity to GSNO. Collectively, our findings suggest Cys-BOOST as a concurrent method of choice for Cys PTM analysis.
HostingRepository	PRIDE
AnnounceDate	2019-05-21
AnnouncementXML	Submission_2019-05-21_05:15:44.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011131
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ruzanna Mnatsakanyan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotin-maleimide; iodoTMT6plex; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-18 06:11:58	ID requested
⏵ 1	2019-05-21 05:15:46	announced

Mnatsakanyan R, Markoutsa S, Walbrunn K, Roos A, Verhelst SHL, Zahedi RP, Proteome-wide detection of S-nitrosylation targets and motifs using bioorthogonal cleavable-linker-based enrichment and switch technique. Nat Commun, 10(1):2195(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Cysteine, PTM, nitrosylation

René P. Zahedi
contact affiliation	Segal Cancer Proteomics Centre,Lady Davis Institute, Jewish General Hospital
contact email	Rene.Zahedi@ladydavis.ca
lab head
Ruzanna Mnatsakanyan
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	ruzanna.mnatsakanyan@gmx.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD011131

PRIDE project URI

• PRIDE
  1. PXD011131
     1. Label: PRIDE project
     2. Name: Proteome-wide detection of cysteine nitrosylation targets and motifs using bio-orthogonal cleavable linker based enrichment and switch technique (Cys-BOOST)