PXD011123

PXD011123 is an original dataset announced via ProteomeXchange.

Title	In-vitro glutathionylation of SMYD2
Description	Glutathionylation is an important post-translational modification and developing tools to identify which cysteine residues on proteins are glutathionylated in response to different physiological conditions is necessary. This project utilizes an approach consisting of azido-glutathione, a single purified protein, and an oxidant to form disulfide bonds between the azide modified glutathione and the cysteine residues of the protein. After this the reaction is quenched with iodoacetamide, subjected to a click reaction with a chemically cleavable alkyne conjugated biotin, and digested with trypsin. These peptide fragments are eluted from streptavidin beads and subjected to LC-MS/MS to determine which cysteine residues are glutathionylated in-vitro.
HostingRepository	PRIDE
AnnounceDate	2019-04-03
AnnouncementXML	Submission_2019-04-03_03:12:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011123
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	garrett vanhecke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Nethylmaleimide; Oxidation
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-18 02:27:37	ID requested
⏵ 1	2019-04-03 03:12:13	announced

Munkanatta Godage DNP, VanHecke GC, Samarasinghe KTG, Feng HZ, Hiske M, Holcomb J, Yang Z, Jin JP, Chung CS, Ahn YH, SMYD2 glutathionylation contributes to degradation of sarcomeric proteins. Nat Commun, 9(1):4341(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, purified protein, glutathionylation, cysteine, click chemistry

Young-Hoon Ahn
contact affiliation	Chemistry, Wayne State University, United States
contact email	yahn@chem.wayne.edu
lab head
garrett vanhecke
contact affiliation	Wayne state university
contact email	cl9183@wayne.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD011123

PRIDE project URI

• PRIDE
  1. PXD011123
     1. Label: PRIDE project
     2. Name: In-vitro glutathionylation of SMYD2