PXD011110

PXD011110 is an original dataset announced via ProteomeXchange.

Title	Wheat and Arabidopsis employ different strategies to optimize the adjustment of photosynthesis to fluctuating light under drought stress
Description	Drought is a major cause of crop yield loss worldwide. Plants can adopt different mechanisms to survive under water deficit, where stomatal closure leads to CO2 limitation. This requires increased dissipation of light energy as heat by non-photochemical quenching (NPQ), changes in the structural configuration of light-harvesting complexes, or changes in the mode of photosynthetic electron flow (i.e. linear vs. cyclic). Under field conditions, where light intensity can change rapidly, this problem becomes even more challenging. Particularly the slow release from photoprotection (NPQ relaxation) was recently shown to cause significant loss of potential yield. We analyzed the acclimation of two different wheat varieties and compared their molecular acclimation strategy to Arabidopsis under moderate drought stress at a slow soil dehydration rate, mimicking realistic field conditions. We monitored their photosynthetic activity under fluctuating light intensities and integrated functional and structural data, based on a detailed analysis of photosynthetic parameters combined with biochemical analysis and unbiased shot-gun proteomics. Contrary to previous models based on the damage of photosynthetic complexes as consequence of drought, we found that plants actively preserve and fine-tune their photosynthetic machinery under drought to adjust the photosynthetic electron transfer to fast changes of light intensity. Strikingly, Arabidopsis and wheat use different molecular strategies for this acclimation. While clear differences in NPQ relaxation and phosphorylation levels of photosynthetic proteins were observed in wheat, Arabidopsis modified the light energy distribution among photosynthetic complexes without changing PSII-LHCII phosphorylation. The fine-tuning of NPQ relaxation can therefore represent a potential trait for crop breeding.
HostingRepository	PRIDE
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2022-03-02_02:53:17.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valentin Roustan
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-17 06:27:03	ID requested
⏵ 1	2022-03-02 02:53:18	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: wheat, arabidopsis, drought, photosynthesis, proteomics

Prof. Dr. Markus Teige
contact affiliation	Departement of Ecogenomics and System Biology, Althanstrasse 14, 1090 Vienna, AUSTRIA
contact email	markus.teige@univie.ac.at
lab head
Valentin Roustan
contact affiliation	University of Vienna
contact email	valentin.roustan@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011110
     1. Label: PRIDE project
     2. Name: Wheat and Arabidopsis employ different strategies to optimize the adjustment of photosynthesis to fluctuating light under drought stress