PXD011107

PXD011107 is an original dataset announced via ProteomeXchange.

Title	MAPPING HEART FIBROSIS: INTEGRATIVE PHOSPHOPROTEOMIC PROFILING OF CLINICAL SAMPLES, ANIMAL AND ORGAN-ON-A-CHIP MODELS
Description	Precision mass spectrometry, animal models and organ-on-a-chip (OOC) systems have emerged as promising experimental strategies to study the molecular mechanisms governing human biology and disease, including the molecular basis of cardiovascular disorders like fibrosis where access to patient samples is limiting and subject to confounding variables. Nevertheless, no systematic comparisons have ever been reported, precluding an objective assessment of cross-platform consistency, performance and bias. Here, we apply and evaluate an integrative mass spectrometry-based platform that allows for quantitative global phospho/proteomic surveys of normal and afflicted tissue from human, mouse and OOC-derived specimens. The applicability and utility of this approach was tested in the context of cardiac fibrosis through comprehensive analyses of fibrotic cardiomyocyte samples from Biowire OOC specimens, and cardiac tissue explants from hypertrophic patients and a mouse genetic model, and show that clinically meaningful biological inferences can be generated by leveraging commonalities and unique attributes generated across each platform.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:48:52.519.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uros Kuzmanov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-17 02:59:38	ID requested
1	2019-11-12 15:08:51	announced
⏵ 2	2024-10-22 04:48:57	announced	2024-10-22: Updated project metadata.

10.14814/phy2.14144;

Hakem Zadeh F, Teng ACT, Kuzmanov U, Chambers PJ, Tupling AR, Gramolini AO, AKAP6 and phospholamban colocalize and interact in HEK-293T cells and primary murine cardiomyocytes. Physiol Rep, 7(14):e14144(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, mouse model, heart, hypertrophy, phosphoproteomic, organ-on-a-chip, fibrosis

Andrew Emili
contact affiliation	University of Toronto, Donnelly Centre; Boston University,
contact email	andrew.emili@utoronto.ca
lab head
Uros Kuzmanov
contact affiliation	University of Toronto
contact email	uros.kuzmanov@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011107

PRIDE project URI

• PRIDE
  1. PXD011107
     1. Label: PRIDE project
     2. Name: MAPPING HEART FIBROSIS: INTEGRATIVE PHOSPHOPROTEOMIC PROFILING OF CLINICAL SAMPLES, ANIMAL AND ORGAN-ON-A-CHIP MODELS