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PXD011107

PXD011107 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMAPPING HEART FIBROSIS: INTEGRATIVE PHOSPHOPROTEOMIC PROFILING OF CLINICAL SAMPLES, ANIMAL AND ORGAN-ON-A-CHIP MODELS
DescriptionPrecision mass spectrometry, animal models and organ-on-a-chip (OOC) systems have emerged as promising experimental strategies to study the molecular mechanisms governing human biology and disease, including the molecular basis of cardiovascular disorders like fibrosis where access to patient samples is limiting and subject to confounding variables. Nevertheless, no systematic comparisons have ever been reported, precluding an objective assessment of cross-platform consistency, performance and bias. Here, we apply and evaluate an integrative mass spectrometry-based platform that allows for quantitative global phospho/proteomic surveys of normal and afflicted tissue from human, mouse and OOC-derived specimens. The applicability and utility of this approach was tested in the context of cardiac fibrosis through comprehensive analyses of fibrotic cardiomyocyte samples from Biowire OOC specimens, and cardiac tissue explants from hypertrophic patients and a mouse genetic model, and show that clinically meaningful biological inferences can be generated by leveraging commonalities and unique attributes generated across each platform.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:48:52.519.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUros Kuzmanov
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap Velos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-17 02:59:38ID requested
12019-11-12 15:08:51announced
22024-10-22 04:48:57announced2024-10-22: Updated project metadata.
Publication List
10.14814/phy2.14144;
Hakem Zadeh F, Teng ACT, Kuzmanov U, Chambers PJ, Tupling AR, Gramolini AO, AKAP6 and phospholamban colocalize and interact in HEK-293T cells and primary murine cardiomyocytes. Physiol Rep, 7(14):e14144(2019) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Human, mouse model, heart, hypertrophy, phosphoproteomic, organ-on-a-chip, fibrosis
Contact List
Andrew Emili
contact affiliationUniversity of Toronto, Donnelly Centre; Boston University,
contact emailandrew.emili@utoronto.ca
lab head
Uros Kuzmanov
contact affiliationUniversity of Toronto
contact emailuros.kuzmanov@utoronto.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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