PXD011107 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MAPPING HEART FIBROSIS: INTEGRATIVE PHOSPHOPROTEOMIC PROFILING OF CLINICAL SAMPLES, ANIMAL AND ORGAN-ON-A-CHIP MODELS |
Description | Precision mass spectrometry, animal models and organ-on-a-chip (OOC) systems have emerged as promising experimental strategies to study the molecular mechanisms governing human biology and disease, including the molecular basis of cardiovascular disorders like fibrosis where access to patient samples is limiting and subject to confounding variables. Nevertheless, no systematic comparisons have ever been reported, precluding an objective assessment of cross-platform consistency, performance and bias. Here, we apply and evaluate an integrative mass spectrometry-based platform that allows for quantitative global phospho/proteomic surveys of normal and afflicted tissue from human, mouse and OOC-derived specimens. The applicability and utility of this approach was tested in the context of cardiac fibrosis through comprehensive analyses of fibrotic cardiomyocyte samples from Biowire OOC specimens, and cardiac tissue explants from hypertrophic patients and a mouse genetic model, and show that clinically meaningful biological inferences can be generated by leveraging commonalities and unique attributes generated across each platform. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:48:52.519.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Uros Kuzmanov |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-17 02:59:38 | ID requested | |
1 | 2019-11-12 15:08:51 | announced | |
⏵ 2 | 2024-10-22 04:48:57 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.14814/phy2.14144; |
Hakem Zadeh F, Teng ACT, Kuzmanov U, Chambers PJ, Tupling AR, Gramolini AO, AKAP6 and phospholamban colocalize and interact in HEK-293T cells and primary murine cardiomyocytes. Physiol Rep, 7(14):e14144(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Human, mouse model, heart, hypertrophy, phosphoproteomic, organ-on-a-chip, fibrosis |
Contact List
Andrew Emili |
contact affiliation | University of Toronto, Donnelly Centre; Boston University, |
contact email | andrew.emili@utoronto.ca |
lab head | |
Uros Kuzmanov |
contact affiliation | University of Toronto |
contact email | uros.kuzmanov@utoronto.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011107
- Label: PRIDE project
- Name: MAPPING HEART FIBROSIS: INTEGRATIVE PHOSPHOPROTEOMIC PROFILING OF CLINICAL SAMPLES, ANIMAL AND ORGAN-ON-A-CHIP MODELS