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PXD011103

PXD011103 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA non-canonical SWI/SNF complex underlies synthetic lethality in cancers driven by BAF complex perturbation
DescriptionMammalian SWI/SNF chromatin remodeling complexes exist in three distinct, final-form assemblies: canonical BAF (cBAF), PBAF, and a newly characterized non-canonical complex, ncBAF. However, their complex-specific targeting on chromatin, functions and roles in disease remain largely unknown. Here, we comprehensively map complex assemblies on chromatin and find that ncBAF uniquely localizes to CTCF sites and promoters. We identified ncBAF subunits as major synthetic lethalities specific to human synovial sarcoma and malignant rhabdoid tumor, which share in common cBAF complex perturbation. Chemical and biological depletion of the BRD9 subunit of ncBAF rapidly attenuates SS and MRT cell proliferation. Notably, in cBAF-perturbed cancers, ncBAF complexes retain localization to CTCF sites and promoters, and maintain gene expression at retained mSWI/SNF sites to support cell proliferation in a manner distinct from fusion oncoprotein-mediated targeting. Taken together, these findings unmask the unique targeting and function of ncBAF complexes and present new cancer-specific therapeutic targets.
HostingRepositoryPRIDE
AnnounceDate2018-11-06
AnnouncementXMLSubmission_2018-11-06_08:43:58.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrew D'Avino
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-17 01:22:30ID requested
12018-11-06 08:43:59announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mSWI/SNF, BAF, chromatin remodeling, BRD7, BRD9, DPF2
Contact List
Cigall Kadoch
contact affiliationDana Farber Cancer Institute
contact emailCigall_Kadoch@dfci.harvard.edu
lab head
Andrew D'Avino
contact affiliationBroad Institute of MIT and Harvard
contact emaildavino@broadinstitute.org
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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