PXD011099

PXD011099 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic, Acetyl-Proteomic, and Metabolomic Analysis of Brown Fat Reveals Key Features of Acetyl-CoA Metabolism During Thermogenesis
Description	Stimulating brown adipose tissue (BAT) energy expenditure could be a therapy for obesity and related metabolic diseases. Achieving this requires a systems-level understanding of the biochemical underpinnings of thermogenesis. To identify novel metabolic features of active BAT, we measured protein abundance, protein acetylation, and metabolite levels in BAT isolated from mice living in their thermoneutral zone or in colder environments. We find that the enzymes which synthesize lipids from cytosolic acetyl-coA are among the most robustly increased proteins after cold acclimation, consistent with recent studies highlighting the importance of anabolic de novo lipogenesis in BAT. In addition, many mitochondrial proteins are hyperacetylated by cold acclimation, including several sites on UCP1, which may have functional relevance. Metabolomics analysis further reveals cold-dependent increases to acetylated carnitine and several amino acids. This BAT multi-omics resource highlights widespread proteomic and metabolic changes linked to acetyl-coA synthesis and utilization that may be useful in unraveling the remarkable metabolic properties of active BAT.
HostingRepository	MassIVE
AnnounceDate	2022-03-14
AnnouncementXML	Submission_2022-03-14_07:52:13.210.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samuel Entwisle
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Acetyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-15 22:43:18	ID requested
⏵ 1	2022-03-14 07:52:13	announced

Publication List

Samuel W. Entwisle, Joan Sanchez-Gurmaches, Robert T. Lawrence, David J. Pedersen, Su Myung Jung, Miguel Martin-Perez, Adilson Guilherme, Michael P. Czech, David A. Guertin, Judit Villen. Cold-Induced Thermogenesis Increases Acetylation on the Brown Fat Proteome and Metabolome. bioRxiv 445718
doi: https://doi.org/10.1101/445718.

Samuel W. Entwisle, Joan Sanchez-Gurmaches, Robert T. Lawrence, David J. Pedersen, Su Myung Jung, Miguel Martin-Perez, Adilson Guilherme, Michael P. Czech, David A. Guertin, Judit Villen. Cold-Induced Thermogenesis Increases Acetylation on the Brown Fat Proteome and Metabolome. bioRxiv 445718

doi: https://doi.org/10.1101/445718.

Keyword List

submitter keyword: Brown fat, Acetylation

submitter keyword: Brown fat, Acetylation

Contact List

Judit Villen
contact affiliation	University of Washington
contact email	jvillen@uw.edu
lab head
Samuel Entwisle
contact affiliation	Harvard Medical School
contact email	samuel_entwisle@hms.harvard.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082951/