PXD011098
PXD011098 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bacterial acetylation and the role of different sugar supplements - carbon overflow |
| Description | Lysine acetylation is thought to provide a mechanism for regulating metabolism in diverse bacteria. Indeed, many studies have shown that the majority of enzymes involved in central metabolism are acetylated and that acetylation can alter enzyme activity. However, the details regarding this regulatory mechanism are still unclear, specifically with regards to the signals inducing lysine acetylation. To better understand this global regulatory mechanism, we profiled changes in lysine acetylation during growth of Escherichia coli on glucose or xylose at both high and low sugar concentrations using label-free mass spectrometry. The goal was to see whether lysine acetylation differed during growth on these two sugars. No significant differences, however, were observed. Rather, the initial sugar concentration was the principal factor governing changes in lysine acetylation, with higher sugar concentrations causing more acetylation. These results suggest that acetylation does not target specific metabolic pathways but rather simply targets with accessible lysines, which may or may not alter enzyme activity. They further suggest that lysine acetylation principally results from conditions that favor accumulation of acetyl phosphate, the principal acetate donor in E. coli. |
| HostingRepository | MassIVE |
| AnnounceDate | 2019-03-11 |
| AnnouncementXML | Submission_2019-03-11_11:10:52.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Birgit Schilling |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
| ModificationList | N6-acetyl-L-lysine |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-09-14 19:07:54 | ID requested | |
| ⏵ 1 | 2019-03-11 11:10:53 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: acetylation, mass spectrometry, glucose, xylose |
Contact List
| Birgit Schilling | |
|---|---|
| contact affiliation | The Buck Institute for Research on Aging |
| contact email | bschilling@buckinstitute.org |
| lab head | |
| Birgit Schilling | |
| contact affiliation | Buck Institute |
| contact email | bschilling@buckinstitute.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082949 |
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