PXD011096

PXD011096 is an original dataset announced via ProteomeXchange.

Title	Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites
Description	In malaria parasites, evolution of parasitism has been linked to functional optimisation. Despite this optimisation, most members of a calcium-dependent protein kinase (CDPK) family show genetic redundancy during erythrocytic proliferation. To identify relationships between phospho-signalling pathways, we here screen 294 genetic interactions among protein kinases in Plasmodium berghei. This reveals a synthetic negative interaction between a hypomorphic allele of the protein kinase G (PKG) and CDPK4 to control erythrocyte invasion which is conserved in P. falciparum. CDPK4 becomes critical when PKG-dependent calcium signals are attenuated to phosphorylate proteins important for the stability of the inner membrane complex, which serves as an anchor for the acto-myosin motor required for motility and invasion. Finally, we show that multiple kinases functionally complement CDPK4 during erythrocytic proliferation and transmission to the mosquito vector. This study reveals how CDPKs are wired within a stage-transcending signalling network to control motility and host cell invasion in malaria parasites.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:48:48.743.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mathieu Brochet
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-14 07:14:59	ID requested
1	2018-09-17 05:08:27	announced
2	2018-11-27 09:19:04	announced	Updated publication reference for PubMed record(s): 30315162.
⏵ 3	2024-10-22 04:48:56	announced	2024-10-22: Updated project metadata.

10.1038/s41467-018-06733-w;

Fang H, Gomes AR, Klages N, Pino P, Maco B, Walker EM, Zenonos ZA, Angrisano F, Baum J, Doerig C, Baker DA, Billker O, Brochet M, Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites. Nat Commun, 9(1):4248(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Plasmodium berghei, schizont, CDPK

Mathieu Brochet
contact affiliation	Department of Molecular Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Switzerland
contact email	Mathieu.Brochet@unige.ch
lab head
Mathieu Brochet
contact affiliation	University of Geneva
contact email	Mathieu.Brochet@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD011096

PRIDE project URI

• PRIDE
  1. PXD011096
     1. Label: PRIDE project
     2. Name: Epistasis studies reveal redundancy among calcium-dependent protein kinases in motility and invasion of malaria parasites