PXD011092

PXD011092 is an original dataset announced via ProteomeXchange.

Title	Crosslinking Mapping of yeast Komagataella phaffii TFIID complex
Description	Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. We used CXMS to study the organization and structure of the purified TFIID complex from Komagataella phaffii. Together with Cryo-EM, We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a HF domain. Interaction with promoter DNA highlights two non-selective binding sites consistent with a DNA scanning mode.
HostingRepository	PRIDE
AnnounceDate	2018-10-08
AnnouncementXML	Submission_2019-05-14_07:21:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Luo
SpeciesList	scientific name: Komagataella phaffii; NCBI TaxID: 460519;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-14 01:45:07	ID requested
1	2018-10-08 05:41:44	announced
⏵ 2	2019-05-14 07:21:46	announced	Updated publication reference for PubMed record(s): 30975984.

Chakraborty M, Tarasovetc EV, Zaytsev AV, Godzi M, Figueiredo AC, Ataullakhanov FI, Grishchuk EL, Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity. Nat Commun, 10(1):1673(2019) [pubmed]

curator keyword: Biological

submitter keyword: K. phaffii TFIID CXMS BS3

Jeff Ranish
contact affiliation	Institute for Systems Biology, Seattle, WA 98109
contact email	jranish@systemsbiology.org
lab head
Jie Luo
contact affiliation	Institute for Systems Biology
contact email	jluo@systemsbiology.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD011092

PRIDE project URI

• PRIDE
  1. PXD011092
     1. Label: PRIDE project
     2. Name: Crosslinking Mapping of yeast Komagataella phaffii TFIID complex