PXD011087 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Shotgun proteomic profiling of intestinal mucosa of rainbow trout Oncorhynchus mykiss |
Description | The objective of this study was to identify and quantify proteomic profiles of intestine of rainbow trout (Oncorhynchus mykiss). Specific pathogen free rainbow trout (mean length 15 ± 1 cm) were maintained in recirculating de-chlorinated water at 19±1 °C. Prior to the experiment, fish were distributed between aquaria. The test groups were infected by immersion of Yersinia ruckeri CSF007-82 (biotype 1) and 7959-11 (biotype 2) strains. The control group was immersed similar with sterile broth medium. Fish were anaesthetized and sampled aseptically at different time points. Each intestine was washed three times with sterile phosphate-buffered saline containing a cocktail of mammalian protease inhibitors. Intestinal mucosa was scraped with a sterile large scalpel blade. Intestinal samples were snap-frozen in liquid nitrogen and stored at –80 °C. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-12 |
AnnouncementXML | Submission_2019-11-12_13:59:22.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gokhlesh Kumar |
SpeciesList | scientific name: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri); NCBI TaxID: 8022; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-14 00:41:22 | ID requested | |
⏵ 1 | 2019-11-12 13:59:24 | announced | |
Publication List
Kumar G, Hummel K, Razzazi-Fazeli E, El-Matbouli M, Modulation of posterior intestinal mucosal proteome in rainbow trout (Oncorhynchus mykiss) after Yersinia ruckeri infection. Vet Res, 50(1):54(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Rainbow trout, intestine, LC MS-MS, Proteome profile |
Contact List
Dr. Gokhlesh Kumar |
contact affiliation | Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria |
contact email | gokhlesh.kumar@vetmeduni.ac.at |
lab head | |
Gokhlesh Kumar |
contact affiliation | University of Veterinary Medicine, Vienna |
contact email | Gokhlesh.Kumar@vetmeduni.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD011087
- Label: PRIDE project
- Name: Shotgun proteomic profiling of intestinal mucosa of rainbow trout Oncorhynchus mykiss