PXD011086

PXD011086 is an original dataset announced via ProteomeXchange.

Title	Analysis of the proteome of individual equine cumulus complexes after maturation in-vitro and in-vivo
Description	Maturation of oocytes under in-vitro conditions (IVM) results in impaired developmental competence compared to oocytes matured in-vivo. Oocytes are closely coupled to their cumulus complex (COC) with a bidirectional exchange of metabolites. Therefore, elucidation of aberrations in cumulus metabolism in-vitro is crucial for a better mimicking of physiological maturation conditions. The aim of this study was the analysis of the equine cumulus cells proteome of single cumulus complexes of metaphase II oocytes matured either under in-vivo (n=8) or in-vitro (n=7) conditions. For in-vivo COC collection mares were slaughtered 30 hours after injection and cumulus complexes from the dominant follicle were harvested for analysis. For in-vitro maturation COCs were recovered from mares out of oestrous and matured for 30 hours in-vitro. COCs were separated in cumulus complexes and oocytes, and only cumulus of successfully matured oocytes was analyzed for this study. All cumulus samples were washed, snap frozen and stored in liquid nitrogen until preparation for analysis.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_13:55:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jasmin Walter
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-14 00:25:47	ID requested
⏵ 1	2019-11-12 13:55:05	announced

Walter J, Huwiler F, Fortes C, Grossmann J, Roschitzki B, Hu J, Naegeli H, Laczko E, Bleul U, Analysis of the equine "cumulome" reveals major metabolic aberrations after maturation in vitro. BMC Genomics, 20(1):588(2019) [pubmed]

curator keyword: Biological

submitter keyword: cumulus cells, LC-MS/MS, IVM, in-vivo, in-vitro

Prof. Dr. Ulrich Bleul
contact affiliation	Clinic of Reproductive Medicine; Vetsuisse Faculty; University of Zurich
contact email	ubleul@vetclinics.uzh.ch
lab head
Jasmin Walter
contact affiliation	Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich
contact email	jwalter@vetclinics.uzh.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011086
     1. Label: PRIDE project
     2. Name: Analysis of the proteome of individual equine cumulus complexes after maturation in-vitro and in-vivo