PXD011084 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cdk1 Gates Cell Cycle-Dependent tRNA Synthesis by Regulating RNA Polymerase III Activity |
Description | tRNA genes are transcribed by RNA polymerase III (RNAPIII). During recent years it has become clear that RNAPIII activity is strictly regulated by the cell in response to environmental cues and the homeostatic status of the cell. However, the molecular mechanisms that control RNAPIII activity to regulate the amplitude of tDNA transcription in normally cycling cells are not well understood. Here, we show that tRNA levels fluctuate during the cell cycle and reveal the underlying molecular mechanism. The cyclin Clb5 recruits the cyclin dependent kinase Cdk1 to tRNA genes to boost tDNA transcription during S phase. At tDNA genes, Cdk1 promotes the recruitment of TFIIIC, stimulates the interaction between TFIIIB and TFIIIC, and increases the dynamics of RNA polymerase III in vivo. Furthermore, we identified Bdp1 as an important Cdk1 substrate in this process. Preventing Bdp1 phosphorylation prevented cell cycle-dependent recruitment of TFIIIC and abolished the cell cycle-induced increase in tDNA transcription. Our findings demonstrate that under optimal growth conditions Cdk1 gates tRNA synthesis in S phase by regulating the RNAPIII machinery, revealing a direct link between the cell cycle and RNAPIII activity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:48:38.541.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joseph Robertson |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-13 05:54:28 | ID requested | |
1 | 2018-10-19 06:49:34 | announced | |
⏵ 2 | 2024-10-22 04:48:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1093/nar/gky846; |
Herrera MC, Chymkowitch P, Robertson JM, Eriksson J, B, ΓΈ, e SO, Alseth I, Enserink JM, Cdk1 gates cell cycle-dependent tRNA synthesis by regulating RNA polymerase III activity. Nucleic Acids Res, 46(22):11698-11711(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: transcription,Cdk1, tRNA, cell cycle |
Contact List
Jorrit Enserink |
contact affiliation | Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway |
contact email | jorrit.enserink@rr-research.no |
lab head | |
Joseph Robertson |
contact affiliation | Oslo University Hospital |
contact email | joseph.mark.robertson@rr-research.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011084
- Label: PRIDE project
- Name: Cdk1 Gates Cell Cycle-Dependent tRNA Synthesis by Regulating RNA Polymerase III Activity