⮝ Full datasets listing

PXD011084

PXD011084 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCdk1 Gates Cell Cycle-Dependent tRNA Synthesis by Regulating RNA Polymerase III Activity
DescriptiontRNA genes are transcribed by RNA polymerase III (RNAPIII). During recent years it has become clear that RNAPIII activity is strictly regulated by the cell in response to environmental cues and the homeostatic status of the cell. However, the molecular mechanisms that control RNAPIII activity to regulate the amplitude of tDNA transcription in normally cycling cells are not well understood. Here, we show that tRNA levels fluctuate during the cell cycle and reveal the underlying molecular mechanism. The cyclin Clb5 recruits the cyclin dependent kinase Cdk1 to tRNA genes to boost tDNA transcription during S phase. At tDNA genes, Cdk1 promotes the recruitment of TFIIIC, stimulates the interaction between TFIIIB and TFIIIC, and increases the dynamics of RNA polymerase III in vivo. Furthermore, we identified Bdp1 as an important Cdk1 substrate in this process. Preventing Bdp1 phosphorylation prevented cell cycle-dependent recruitment of TFIIIC and abolished the cell cycle-induced increase in tDNA transcription. Our findings demonstrate that under optimal growth conditions Cdk1 gates tRNA synthesis in S phase by regulating the RNAPIII machinery, revealing a direct link between the cell cycle and RNAPIII activity.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:48:38.541.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJoseph Robertson
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListphosphorylated residue; acetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-13 05:54:28ID requested
12018-10-19 06:49:34announced
22024-10-22 04:48:39announced2024-10-22: Updated project metadata.
Publication List
10.1093/nar/gky846;
Herrera MC, Chymkowitch P, Robertson JM, Eriksson J, B, ΓΈ, e SO, Alseth I, Enserink JM, Cdk1 gates cell cycle-dependent tRNA synthesis by regulating RNA polymerase III activity. Nucleic Acids Res, 46(22):11698-11711(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: transcription,Cdk1, tRNA, cell cycle
Contact List
Jorrit Enserink
contact affiliationDepartment of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway
contact emailjorrit.enserink@rr-research.no
lab head
Joseph Robertson
contact affiliationOslo University Hospital
contact emailjoseph.mark.robertson@rr-research.no
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD011084
PRIDE project URI
Repository Record List
[ + ]