PXD011081

PXD011081 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Mycobacterium tuberculosis Protein-Mannosyltransferase Knock-Out Mutant.
Description	This project focused in the proteomic characterization of the secreted proteome of a Rv1002c (Protein mannosyltransferase) knock-out mutant and its wild type counterpart. The analysis was carried out using a M. tuberculosis ΔleuDΔpanCD auxotroph Rv1002c wild type compared to M. tuberculosis ΔleuDΔpanCDΔRv1002c. Both strains were grown in triplicate and their whole cell lysate (WCL) and secreted proteins (CFP) where purified, trypsin digested and characterized by liquid-chromatography coupled with tandem mass spectrometry (LC-MS/MS). Three replicate injections were performed for each of the sampels. The resulting spectra were searched against an M. tuberculosis database and relative protein abundance was determined using normalized spectral counts. Protein abundance differences were tested by Student’s t test. Abundance of 39 secreted proteins was significantly different between the WT and the ΔRv1002c. Decrease abundance of known glycoproteins, including several lipoglycoproteins was observed in the ΔRv1002c strain. In the WCL, 61 proteins presented significantly different abundance between the WT and the ΔRv1002c strains, including 4 proteins that were completely absent in the mutant (Rv2510c, PknF, Mpt70 and CeoC).
HostingRepository	PRIDE
AnnounceDate	2019-04-12
AnnouncementXML	Submission_2019-04-12_01:13:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011081
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Carolina Mehaffy
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-13 03:54:25	ID requested
⏵ 1	2019-04-12 01:13:23	announced

Mehaffy C, Belisle JT, Dobos KM, Mycobacteria and their sweet proteins: An overview of protein glycosylation and lipoglycosylation in M. tuberculosis. Tuberculosis (Edinb), 115():1-13(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Proteomics, Mycobaterium, tuberculosis, protein glycosylation

Carolina Mehaffy
contact affiliation	Department of Microbiology, Immunology and Pathology. Colorado State University, 1682 Campus delivery, Fort Collins, CO, USA
contact email	carolina.mehaffy@colostate.edu
lab head
Carolina Mehaffy
contact affiliation	Colorado State University
contact email	carolina.mehaffy@colostate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD011081

PRIDE project URI

• PRIDE
  1. PXD011081
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Mycobacterium tuberculosis Protein-Mannosyltransferase Knock-Out Mutant.