PXD011078

PXD011078 is an original dataset announced via ProteomeXchange.

Title	The Bloom’s syndrome helicase BLM functions as a homodimer within the Bloom’s complex
Description	Homologous recombination (HR) is an essential and highly regulated cellular process. Unintended HR in somatic cells is potentially deleterious; it can result in translocations and/or somatic cell loss of heterozygosity. This is suppressed by the BLM protein in combination with topoisomerase III, RMI1 and RMI2 in a hetero-tetrameric complex (the ‘Bloom’s complex’). To understand how the activities of BLM helicase and topoisomerase III are coupled, we purified the four-subunit complex. Chemical crosslinking and mass spectrometry revealed a unique architecture that couples helicase and topoisomerase domains.
HostingRepository	PRIDE
AnnounceDate	2022-01-17
AnnouncementXML	Submission_2022-01-17_03:36:59.764.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jason Low
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-13 02:00:33	ID requested
⏵ 1	2022-01-17 03:37:00	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: BLM, Bloom’s Syndrome, crosslinking MS, structural proteomics

Andrew J Deans
contact affiliation	Genome Stability Unit, St. Vincent’s Institute of Medical Research, Fitzroy, Australia
contact email	adeans@svi.edu.au
lab head
Jason Low
contact affiliation	The University of Sydney
contact email	jason.low@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD011078

PRIDE project URI

• PRIDE
  1. PXD011078
     1. Label: PRIDE project
     2. Name: The Bloom’s syndrome helicase BLM functions as a homodimer within the Bloom’s complex