PXD011075

PXD011075 is an original dataset announced via ProteomeXchange.

Title	Human tear fluid proteome dataset for usage as a spectral library and for protein modeling
Description	This article provides a detailed dataset of human tear fluid proteins. Samples were fractionated by sodium dodecyl sulfate (SDS) gel electrophoresis resulting in 48 fractions that were spiked with an indexed retention time (iRT) peptide standard. These data are based on a data-dependent acquisition (DDA) mass spectrometric approach and can be used for example as a spectral library for tear fluid proteome analysis by data-independent acquisition (DIA). Moreover, the provided data set can be used with optimized HPLC as well as mass spectrometric settings for proteins/peptides of interest. Besides these aspects, this dataset can serve as a protein overview for gene ontology enrichment analysis as well as for modeling and benchmarking of multiple signaling pathways associated with the ocular surface in healthy or disease stages.
HostingRepository	PRIDE
AnnounceDate	2019-08-15
AnnouncementXML	Submission_2019-08-15_01:12:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annika Guntermann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-13 00:01:19	ID requested
⏵ 1	2019-08-15 01:18:22	announced

Guntermann A, Steinbach S, Serschnitzki B, Grotegut P, Reinehr S, Joachim SC, Schargus M, Marcus K, May C, Human tear fluid proteome dataset for usage as a spectral library and for protein modeling. Data Brief, 23():103742(2019) [pubmed]

ProteomeXchange project tag: Human Brain Proteome Project (HUPO_HBPP) (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

curator keyword: Biological

submitter keyword: tear fluid, LC-MS/MS, spectral library, DDA, DIA, protein modeling

Prof. Dr. Katrin Marcus
contact affiliation	Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany
contact email	katrin.marcus@rub.de
lab head
Annika Guntermann
contact affiliation	Medizinisches Proteom-Center
contact email	Annika.Guntermann@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD011075

PRIDE project URI

• PRIDE
  1. PXD011075
     1. Label: PRIDE project
     2. Name: Human tear fluid proteome dataset for usage as a spectral library and for protein modeling