PXD011060

PXD011060 is an original dataset announced via ProteomeXchange.

Title	HDX-MS on secondary transporters
Description	Secondary transporters undergo structural rearrangements to catalyze substrate translocation across the cell membrane – yet how such conformational changes happen within a lipid environment remains poorly understood. Here, we combine hydrogen-deuterium exchange mass spectrometry (HDX-MS) with molecular dynamics (MD) simulations to understand how lipids regulate the conformational dynamics of secondary transporters at the molecular level. Using the homologous transporters XylE, LacY and GlpT from Escherichia coli as model systems, we discover that conserved networks of charged residues act as molecular switches that drive the conformational transition between different states. We reveal that these molecular switches are regulated by interactions with surrounding phospholipids and show that phosphatidylethanolamine interferes with the formation of the conserved networks and favors an inward-facing state. Overall, this work provides insights into the importance of lipids in shaping the conformational landscape of an important class of transporters.
HostingRepository	PRIDE
AnnounceDate	2018-10-16
AnnouncementXML	Submission_2018-10-15_21:33:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chloe Martens
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-11 06:48:24	ID requested
⏵ 1	2018-10-15 21:33:10	announced

Martens C, Shekhar M, Borysik AJ, Lau AM, Reading E, Tajkhorshid E, Booth PJ, Politis A, Direct protein-lipid interactions shape the conformational landscape of secondary transporters. Nat Commun, 9(1):4151(2018) [pubmed]

curator keyword: Biological

submitter keyword: conformational dynamics, lipid-protein interactions, structural biology, membrane proteins

Argyris Politis
contact affiliation	King's College London Chemistry department 7 Trinity street SE1 1DB London UK
contact email	argyris.politis@kcl.ac.uk
lab head
Chloe Martens
contact affiliation	king's college london
contact email	chloe.martens@kcl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD011060

PRIDE project URI

• PRIDE
  1. PXD011060
     1. Label: PRIDE project
     2. Name: HDX-MS on secondary transporters