PXD011059

PXD011059 is an original dataset announced via ProteomeXchange.

Title	Proteomic changes induced by cold challenge in gilthead sea bream (Sparus aurata) liver
Description	We report the proteomic characterization of livers from Sparus aurata exposed to cold temperatures. In this study, mimicking the winter challenge conditions, a 8 week feeding trial was carried out on gilthead sea bream juveniles reared in RAS systems at a temperature ramp made of two phases of four weeks each: a cooling phase from 18°C 8 (t0) to 11°C (t1) and a cold maintenance phase at 11°C (t2). Sparus aurata livers, after exposure to the three temperature phases (t0, t1 and t2), were collected and analyzed using a shotgun proteomics approach based on filter-aided sample preparation followed by tandem mass spectrometry, peptide identification carried out using Sequest-HT as search engine within the Proteome Discoverer informatic platform, and label-free differential analysis. Along the whole trial, sea breams underwent several changes occurring upon thermal stress in liver protein abundance. These occurred mostly during the cooling phase, when catabolic processes were mainly observed. These included protein and lipid degradation and a decrease in protein synthesis and amino acid metabolism. A decrease in protein mediators of oxidative stress protection was also seen. Liver protein profiles showed less marked changes during cold maintenance, although pathways such as the methionine cycle and sugar metabolism were significantly affected. This study provided useful hints on the dynamics and extent of the metabolic shift occurring in sea bream liver with decreasing water temperature, helping the development of feeds aimed at compensating the thermal stress encountered by fish in offshore farming conditions.
HostingRepository	PRIDE
AnnounceDate	2019-05-28
AnnouncementXML	Submission_2019-05-31_02:35:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefania Ghisaura
SpeciesList	scientific name: Sparus aurata; NCBI TaxID: 8175;
ModificationList	iodoacetamide derivatized residue
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-11 06:18:30	ID requested
1	2019-05-28 02:09:46	announced
⏵ 2	2019-05-31 02:35:08	announced	Updated publication reference for PubMed record(s): 31128654.

Ghisaura S, Pagnozzi D, Melis R, Biosa G, Slawski H, Uzzau S, Anedda R, Addis MF, Liver proteomics of gilthead sea bream (Sparus aurata) exposed to cold stress. J Therm Biol, 82():234-241(2019) [pubmed]

submitter keyword: Sparus aurata, liver, proteomics, LC-MS/MS, cold exposure

Daniela Pagnozzi
contact affiliation	Porto Conte Ricerche S.r.l.
contact email	pagnozzi@portocontericerche.it
lab head
Stefania Ghisaura
contact affiliation	Porto Conte Ricerche S.r.l.
contact email	ghisaura@portocontericerche.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011059
     1. Label: PRIDE project
     2. Name: Proteomic changes induced by cold challenge in gilthead sea bream (Sparus aurata) liver